McAndrews J M, Ringstrom S J, Dahl K D, Schwartz N B
Department of Neurobiology and Physiology, Northwestern University, 60208, Evanston, Illinois, U.S.A..
Endocrine. 1995 Jan;3(1):13-20. doi: 10.1007/BF02917443.
The effects of corticosterone (B) and testosterone (T) on pituitary and serum bioactive and immunoreactive gonadotropins and on gonadotropin hormone subunit messenger RNA levels were compared in the absence of GnRH. Male rats were implanted with pellets of either cholesterol, B or T. At implantation, 2 and 4 days later half of each group received GnRH antagonist and animals were killed 5 days after implantation. As expected, GnRH antagonist lowered bioactive and immunoreactive serum FSH and LH, pituitary FSH, LHβ and FSHβ mRNA. B treatment alone lowered bioactive and immunoreactive serum FSH and immunoreactive serum LH. B reversed the antagonist effect on bioactive and immunoreactive pituitary FSH and FSHβ mRNA. T alone lowered bioactive and immunoreactive serum FSH and LH levels. T reversed the antagonist effect on bioactive and immunoreactive pituitary FSH. T lowered bioactive and immunoreactive pituitary LH and LHβ mRNA and partially reversed the antagonist effect on FSHβ mRNA. The data suggest that either B or T enhance FSH synthesis by acting directly at the gonadotrope, but that B does not affect LH variables to the same extent as T. The results suggest that in stressed animals, when T levels are reduced, B can substitute for T in sustaining FSH synthesis.
在不存在促性腺激素释放激素（GnRH）的情况下，比较了皮质酮（B）和睾酮（T）对垂体及血清中生物活性和免疫反应性促性腺激素以及促性腺激素亚基信使核糖核酸（mRNA）水平的影响。给雄性大鼠植入胆固醇、B或T的丸剂。在植入时、植入后2天和4天，每组的一半动物接受GnRH拮抗剂，动物在植入后5天处死。正如预期的那样，GnRH拮抗剂降低了生物活性和免疫反应性血清促卵泡激素（FSH）和促黄体生成素（LH）、垂体FSH、LHβ和FSHβ mRNA。单独使用B降低了生物活性和免疫反应性血清FSH以及免疫反应性血清LH。B逆转了拮抗剂对生物活性和免疫反应性垂体FSH以及FSHβ mRNA的作用。单独使用T降低了生物活性和免疫反应性血清FSH和LH水平。T逆转了拮抗剂对生物活性和免疫反应性垂体FSH的作用。T降低了生物活性和免疫反应性垂体LH和LHβ mRNA，并部分逆转了拮抗剂对FSHβ mRNA的作用。数据表明，B或T通过直接作用于促性腺细胞来增强FSH合成，但B对LH变量的影响程度与T不同。结果表明，在应激动物中，当T水平降低时，B可以替代T来维持FSH合成。
