Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Mol Biol Rep. 2011 Nov;38(8):4873-7. doi: 10.1007/s11033-010-0628-7. Epub 2010 Dec 14.
Research on the frequency of the highly functional mutations of genes coding required for metabolizing enzymes has shown significant ethnic variations. However, few studies, if any, have examined the frequency distribution of major allelic variations in the context of Iran. In this regard, the present study focused on the genotype profile of Southern Iranians in order to compare allele frequencies of their CYP3A5 and P2Y12 (T744C) which have been shown to have roles in metabolizing clopidogrel, with those of other populations. Therefore, genotyping was carried out on 112 unrelated individuals by PCR-RFLP. The CYP3A5*3 allele was found in 185 persons with allelic frequency 0.82, which is the most common allele among Caucasians (90-95%). The frequency of 82% is different from other Caucasians (90-94%), Indians (67%), Vietnam (67%) and Africans (15%). but lower than frequency in Chinese populations (74%) and Korean (76%). The allele frequency of the -744T (4%) is different from frequencies of Caucasian, American, Chinese, Korean, and Subsahara population. This study confirmed significant inter-ethnic differences in CYP3A5 and P2Y12 frequencies between Iranians and other ethnic groups. The results of this study will be useful for clinical pharmacokinetic investigations and drug dosage recommendations especially antiplatelet drugs such as Clopidogrel, for Iranians.
研究表明，编码代谢酶所需的基因的高功能突变的频率存在显著的种族差异。然而，很少有研究（如果有的话）在伊朗的背景下研究主要等位基因变异的频率分布。在这方面，本研究集中于对伊朗南部人群的基因型谱进行研究，以便比较其 CYP3A5 和 P2Y12（T744C）等位基因频率，这些基因已被证明在代谢氯吡格雷方面发挥作用，与其他人群的等位基因频率进行比较。因此，通过 PCR-RFLP 对 112 名无关个体进行了基因分型。在 185 名个体中发现了 CYP3A5*3 等位基因，等位基因频率为 0.82，这是白种人中最常见的等位基因（90-95%）。82%的频率与其他白种人（90-94%）、印度人（67%）、越南人（67%）和非洲人（15%）不同，但低于中国人（74%）和韩国人（76%）的频率。-744T（4%）等位基因频率与白种人、美国人、中国人、韩国人和撒哈拉以南人群的频率不同。本研究证实了伊朗人与其他种族之间 CYP3A5 和 P2Y12 频率存在显著的种族差异。这些结果将有助于对伊朗人进行临床药代动力学研究和药物剂量建议，特别是氯吡格雷等抗血小板药物。
