Vargas-Alarcón Gilberto, Ramírez-Bello Julián, de la Peña Aurora, Calderón-Cruz Beatriz, Peña-Duque Marco Antonio, Martínez-Ríos Marco Antonio, Ramírez-Fuentes Silvestre, Pérez-Méndez Oscar, Fragoso José Manuel
Department of Molecular Biology, Instituto Nacional de Cardiología Ignacio Chávez, Juan Badiano No. 1, Tlalpan, 14080, Mexico City, DF, Mexico.
Mol Biol Rep. 2014 Oct;41(10):7023-9. doi: 10.1007/s11033-014-3590-y. Epub 2014 Aug 9.
The aim of the present study was to establish the gene frequency of six polymorphisms of the ABCB1, CYP3A5, CYP2C19, and P2RY12 genes in a population resident of Mexico City. The proteins encoded by these genes have been associated with the absorption, and biotransformation of clopidogrel. The ABCB1 T3435C, CYP3A5 V3 A6986G, P2RY12 G52T, P2RY12 C34T, CYP2C19 V2 and V3 (positions G681A and G636A, respectively), polymorphisms were analyzed by 5' exonuclease TaqMan genotyping assays in a group of 269 healthy unrelated Mexican Mestizo individuals. The CYP2C19 V3 G636A polymorphism was not detected in the Mexican Mestizos population. However, the studied population presented significant differences (P < 0.05) in the distribution of the T3435C, A6986G, G681A, G52T and C34T polymorphisms when compared to reported frequencies of Amerindian of South America, Caucasian, Asian, and African populations. In summary, the distribution of the ABCB1, CYP3A5, CYP2C19, and P2RY12 gene polymorphisms distinguishes to the Mexican Mestizos population from other ethnic groups.
本研究的目的是确定墨西哥城居民群体中ABCB1、CYP3A5、CYP2C19和P2RY12基因六种多态性的基因频率。这些基因编码的蛋白质与氯吡格雷的吸收和生物转化有关。通过5'核酸外切酶TaqMan基因分型检测法，对一组269名健康、无亲缘关系的墨西哥梅斯蒂索个体进行了ABCB1 T3435C、CYP3A5 V3 A6986G、P2RY12 G52T、P2RY12 C34T、CYP2C19 V2和V3（分别为G681A和G636A位点）多态性分析。在墨西哥梅斯蒂索人群中未检测到CYP2C19 V3 G636A多态性。然而，与南美洲美洲印第安人、白种人、亚洲人和非洲人群的报告频率相比，所研究人群在T3435C、A6986G、G681A、G52T和C34T多态性的分布上存在显著差异（P < 0.05）。总之，ABCB1、CYP3A5、CYP2C19和P2RY12基因多态性的分布使墨西哥梅斯蒂索人群与其他种族群体区分开来。
