Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St Paul, MN 55108, USA.
Avian Pathol. 2010 Dec;39(6):441-5. doi: 10.1080/03079457.2010.517249.
The present study was undertaken to detect and characterize Newcastle disease virus (NDV) in raptors. Cloacal and oropharyngeal swab samples were collected from 60 casualty raptors during January to March 2009 in Minnesota. Inoculation of all these samples (n=120) in 9-day-old embryonated hens' eggs resulted in isolation of haemagglutinating viruses in three samples from two bald eagles and one great horned owl. These three haemagglutinating viruses were confirmed as NDV by reverse transcription-polymerase chain reaction (RT-PCR) using fusion gene-specific primers, and were negative for avian influenza virus by RT-PCR. Further characterization revealed that all three possessed (112)GKQGRL(117) at the fusion gene cleavage site, indicating that they were lentogenic strains. Phylogenetic analysis revealed that all three isolates clustered with published class II genotype II NDVs. The nucleotide sequence homology of the three NDV isolates among themselves was 98.4 to 99.6% and the sequence homology with lentogenic strains from wild birds used for comparison varied between 94.5 and 100%. Detection of NDV strains from raptors merits further epidemiological studies to determine the prevalence of different NDV strains in raptors and their impact in relation to transmission to domestic poultry.
本研究旨在检测和鉴定猛禽中的新城疫病毒（NDV）。2009 年 1 月至 3 月，从明尼苏达州的 60 只受伤猛禽中采集了泄殖腔和口咽拭子样本。将所有这些样本（n=120）接种到 9 日龄的鸡胚中，从两只白头海雕和一只大角猫头鹰的三个样本中分离出了具有血凝性的病毒。这三种血凝病毒通过使用融合基因特异性引物的反转录-聚合酶链反应（RT-PCR）被确认为 NDV，并通过 RT-PCR 对禽流感病毒呈阴性。进一步的特征分析表明，所有三种病毒在融合基因切割位点都具有（112）GKQGRL（117），表明它们是低致病性毒株。系统进化分析显示，这三种分离株与已发表的 II 类基因型 II NDV 聚为一簇。三种 NDV 分离株之间的核苷酸序列同源性为 98.4%至 99.6%，与用于比较的野生鸟类中的低致病性株的序列同源性在 94.5%至 100%之间。从猛禽中检测到 NDV 株值得进一步进行流行病学研究，以确定不同 NDV 株在猛禽中的流行情况及其与向家禽传播的关系。
