Department of Chemistry and Center for Electro-Photo Behaviors in Advanced Molecular Systems, Pohang University of Science and Technology (POSTECH), San 31 Hyoja-dong, Pohang 790-784, Korea.
Chem Asian J. 2011 Jan 3;6(1):122-7. doi: 10.1002/asia.201000621.
A microarray-chip assay system for the fluorescence detection of phosphate-containing analytes in aqueous media has been constructed from stimuli-responsive polymerized poly(diacetylene)-liposomes for the first time. Proper combination of the liposome components (Zn(II)-dipicolylamine for phosphate binding and an amine-terminated component for anchoring the liposome onto an aldehyde-derivatized glass plate), has led to a microarray chip that selectively detects pyrophosphate, an important biomarker, over competing anions, such as phosphate and adenosine triphosphate, with nanomolar sensitivity. The chip-based assay shows advantages, such as high specificity and sensitivity, over solution-based assays that use the same liposomes, and over known homogeneous molecular sensing systems.
首次构建了一种基于刺激响应聚合物化聚二乙炔脂双层囊泡的用于在水介质中荧光检测含磷分析物的微阵列芯片检测系统。脂双层囊泡组件（用于结合磷酸盐的 Zn(II)-二吡啶甲胺和用于将脂双层囊泡固定在醛基衍生化玻璃片上的胺端组件）的合理组合，导致了一种微阵列芯片，该芯片对焦磷酸盐（一种重要的生物标志物）具有选择性，对竞争阴离子（如磷酸盐和三磷酸腺苷）的检测具有纳米级灵敏度。与使用相同脂双层囊泡的基于溶液的测定法以及已知的均相分子传感系统相比，基于芯片的测定法具有高特异性和灵敏度等优点。
