[A new expressible VH-gene of the 36-40 family participates in the biosynthesis of antibodies against swine transferrin].

The expressible allelic variant of immunoglobulin heavy chain genes locus from hybridoma PTF-02 is localized in the 17 kb Bgl II fragment of DNA. The structure of VDJh 3 exon and flanking regions was investigated. The 1101 bp fragment was sequenced. It contains transcription regulatory sequences (cd and TTAAAT-box), the leader exon with internal intron (L), the Vh exon, D and Jh 3 segments. The Vh exon has unusual for immunoglobulin genes the first codon-threonine codon. It was shown that expressible in hybridoma PTF-02 Vh gene (Vh TF) represents and earlier unknown gene of the mouse 36-60 Vh family. This conclusion was made after the comparison of Vh TF exon structure and its 5'-region physical map with the other genes of the 36-60 family.