Halquist Matthew S, Thomas Karnes H
Virginia Commonwealth University, Department of Pharmaceutics, PO Box 980533, Richmond, Virginia 980533, USA.
Biomed Chromatogr. 2011 Jan;25(1-2):47-58. doi: 10.1002/bmc.1545. Epub 2010 Dec 10.
Targeted protein quantification using peptide surrogates has increasingly become important to the validation of biomarker candidates and development of protein therapeutics. These approaches have been proposed and employed as alternatives to immunoassays in biological fluids. Technological advances over the last 20 years in biochemistry and mass spectrometry have prompted the use of peptides as surrogates to quantify enzyme digested proteins using triple quadrupole mass spectrometers. Multiple sample preparation processes are often incorporated to achieve quantification of target proteins using these signature peptides. This review article focuses on these processes or hyphenated techniques for quantification of proteins with peptide surrogates. The most recent advances and strategies involved with hyphenated techniques are discussed.
使用肽替代物进行靶向蛋白质定量，对于生物标志物候选物的验证和蛋白质治疗药物的开发日益重要。这些方法已被提出并用作生物体液中免疫测定的替代方法。过去20年生物化学和质谱技术的进步促使人们使用肽作为替代物，通过三重四极杆质谱仪对酶解蛋白质进行定量。为了使用这些特征肽实现目标蛋白质的定量，通常会采用多种样品制备过程。这篇综述文章重点关注这些使用肽替代物进行蛋白质定量的过程或联用技术。文中讨论了联用技术涉及的最新进展和策略。
