Luo Qian-qian, Yang Jun-xia, Zhang Xiao-min, Li Jie-jia, Shao Jia-xin, Zhu Li
Institute of Nautical Medicine, Nantong University, Nantong 226001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Aug;25(3):362-5.
To investigate the protective effects of ginkgolide B and hypoxic preconditioning against acute hypoxia injury in mice.
Ordinary pressure acute hypoxia model in mice was adopted to observe the ethology, the duration of the death and the degree of brain edema. Meanwhile the expression of RTP801 mRNA and erythropoietin (EPO) were measured by RT-PCR and Western blot, respectively.
Ginkgolide B and hypoxic preconditioning could both prolong the survival time of hypoxia under ordinary pressure,and significantly decreased the degree of brain edema. Besides ginkgolide B and hypoxic preconditioning could both up-regulate the expression of RTP801mRNA and EPO.
Ginkgolide B has the similar effect to hypoxic preconditioning against acute hypoxia. Both of these protective effects may be associated with the up-regulation of the expression of RTP801 mRNA and EPO.
探讨银杏内酯B及缺氧预处理对小鼠急性缺氧损伤的保护作用。
采用小鼠常压急性缺氧模型，观察其行为学、死亡持续时间及脑水肿程度。同时，分别采用逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法（Western blot）检测RTP801信使核糖核酸（mRNA）及促红细胞生成素（EPO）的表达。
银杏内酯B及缺氧预处理均可延长常压缺氧小鼠的存活时间，并显著减轻脑水肿程度。此外，银杏内酯B及缺氧预处理均可上调RTP801mRNA及EPO的表达。
银杏内酯B在对抗急性缺氧方面具有与缺氧预处理相似的作用。这两种保护作用可能均与RTP801mRNA及EPO表达上调有关。
