Cao Bei-Bei, Peng Yu-Ping, Qiu Yi-Hua
Department of Physiology, School of Basic Medical Sciences, Nantong University, Nantong 226001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 Nov;22(4):410-4.
To investigate effect of cerebellar fastigial nuclei (FN), one of three deep nuclei in cerebellum, on lymphocyte function, and possible central pathway involved in the effect.
Kainic acid (KA) was microinjected into bilateral FN of rats. On the eighth day after the surgery, lymphocytes from the mesenteric lymph nodes were incubated to measure their proliferative reaction to concanavalin A (Con A) by means of colorimetric assay of methyl thiazole tetrazolium (MTT), and natural killer (NK) cells from the spleen were cultured to evaluate their cytotoxicity to YAC-1 cells with the aid of flow cytometric assay. Simultaneously, glutamate content in the hypothalamus was determined by high performance liquid chromatography (HPLC). As control, 0.9% saline was microinjected into the bilateral FN of rats. When these experiments ended, cerebellar sections and Nissl stain for each rat were made to observe the location and extent of the lesions. If the lesion areas were not in the bilateral FN or not limited in the FN, the results were discarded.
On day 8 following the KA injection of FN, the Nissl-stained cerebellar sections showed the neuronal bodies in the FN were effectively damaged by KA. Simultaneously, the lymphocyte proliferation induced by Con A was significantly increased and the NK cell cytotoxicity to YAC-1 target cells was remarkably enhanced when compared with those of the control animals microinjected with saline in their bilateral FN. At the same time as these changes of lymphocyte functions occurred, glutamate content in the hypothalamus was markedly reduced relative to that in the control hypothalamus.
Effective lesions of cerebellar bilateral FN of rats can cause an enhancement of lymphocyte functions, including increase of proliferation of T cells and cytotoxicity of NK cells. The cerebello hypothalamic glutamatergic projections may be involved in the pathway of cerebellar FN immunomodulation.
研究小脑深部核团之一的小脑顶核（FN）对淋巴细胞功能的影响以及该效应可能涉及的中枢通路。
将海藻酸（KA）微量注射到大鼠双侧FN。术后第8天，培养肠系膜淋巴结的淋巴细胞，采用甲基噻唑四氮唑（MTT）比色法检测其对刀豆蛋白A（Con A）的增殖反应，培养脾脏的自然杀伤（NK）细胞，借助流式细胞术检测其对YAC-1细胞的细胞毒性。同时，采用高效液相色谱法（HPLC）测定下丘脑谷氨酸含量。作为对照，将0.9%生理盐水微量注射到大鼠双侧FN。实验结束后，对每只大鼠制作小脑切片并进行尼氏染色，观察损伤的位置和范围。若损伤区域不在双侧FN或不限于FN，则舍弃结果。
FN注射KA后第8天，尼氏染色的小脑切片显示FN中的神经元胞体被KA有效损伤。同时，与双侧FN注射生理盐水的对照动物相比，Con A诱导的淋巴细胞增殖显著增加，NK细胞对YAC-1靶细胞的细胞毒性明显增强。在淋巴细胞功能发生这些变化的同时，下丘脑谷氨酸含量相对于对照下丘脑明显降低。
大鼠双侧小脑FN的有效损伤可导致淋巴细胞功能增强，包括T细胞增殖增加和NK细胞细胞毒性增强。小脑-下丘脑谷氨酸能投射可能参与小脑FN免疫调节通路。
