Liu Dan-Dan, Wang Yue-Zeng, Zhao Dong-Hai, Li Yu-Lin
Key Laboratory of Pathobiology, Jilin University, Changchun 130021, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 Nov;22(4):423-8.
To analyze gene expression difference between human mesenchymal stem cells and umbilical vein endothelial cells, to discuss the feasibility of inducing hMSCs to differentiate into endothelial cells through in vitro gene transfection as well as the use and prospective as a seeding cell source of vascular tissue engineering.
hMSCs and hUVECs were isolated, expanded in culture, and characterized by flow-cytometry, immunocytochemistry, immunofluorescence and transmission electron microscopy (TEM). Differential analysis of gene expression profiles between them was performed by Biostar H-40 cDNA microarrays. The properties of VEGF 165 transfected were also detected with RT-PCR, ELISA et al.
Nearly 86% genes were coexpressed in both cells and hMSCs expressed typical endothelial antigen marker of EC. After VEGF165 transfection, hMSCs (or committed hMSCs) were positive for CD31. To different extent, the expression of CD44 was down regulated and CD34, FVIIIAg, Flt-1 up regulated.
Generation of functional EC or tissue engineered blood vessels from human MSCs is feasible utilizing an in vitro environment gene transfection.
分析人间充质干细胞与脐静脉内皮细胞之间的基因表达差异，探讨通过体外基因转染诱导人骨髓间充质干细胞分化为内皮细胞的可行性以及作为血管组织工程种子细胞来源的应用和前景。
分离、培养扩增人骨髓间充质干细胞和人脐静脉内皮细胞，并通过流式细胞术、免疫细胞化学、免疫荧光及透射电子显微镜（TEM）进行鉴定。采用博奥生物H-40 cDNA芯片对两者之间的基因表达谱进行差异分析。同时应用逆转录聚合酶链反应（RT-PCR）、酶联免疫吸附测定（ELISA）等方法检测转染血管内皮生长因子165（VEGF 165）后的特性。
近86%的基因在两种细胞中共同表达，人骨髓间充质干细胞表达典型的内皮细胞抗原标志物。转染VEGF165后，人骨髓间充质干细胞（或定向分化的人骨髓间充质干细胞）CD31呈阳性。CD44表达不同程度下调，CD34、FVIIIAg、Flt-1表达上调。
利用体外环境基因转染从人骨髓间充质干细胞生成功能性内皮细胞或组织工程血管是可行的。
