Misener V, Jongstra-Bilen J, Young A J, Atkinson M J, Wu G E, Jongstra J
Department of Immunology, University of Toronto, Ontario, Canada.
J Immunol. 1990 Aug 1;145(3):905-9.
Using a polyclonal rabbit antiserum against recombinant mouse lambda 5 protein, we determined that the pre-B cell specific mouse lambda 5 gene encodes a 22-kDa protein. The lambda 5 protein, which is related to conventional Ig lambda L chain proteins forms a complex with Ig mu H chain protein and an as yet unidentified 16-kDa protein (p16) in mu+ pre-B cell lines carrying a functionally rearranged VH-DH-JH allele. In pre-B cell lines which carry DH-JH rearrangements and do not express mu H chain protein, lambda 5 protein is associated with p16. Thus the expression of lambda 5 protein precedes the expression of intact mu H chain protein. This suggests the existence of developmentally regulated protein complexes involving the Ig L chain-like protein lambda 5 and p16 in mu- pre-B cells; lambda 5, p16, and Ig H chain protein in mu+ pre-B cells and Ig H chain and conventional Ig L chain proteins in B cells and plasma cells.
使用针对重组小鼠λ5蛋白的多克隆兔抗血清，我们确定前B细胞特异性小鼠λ5基因编码一种22 kDa的蛋白质。λ5蛋白与传统的Igλ轻链蛋白相关，在携带功能性重排的VH-DH-JH等位基因的μ+前B细胞系中，它与Igμ重链蛋白和一种尚未鉴定的16 kDa蛋白（p16）形成复合物。在携带DH-JH重排且不表达μ重链蛋白的前B细胞系中，λ5蛋白与p16相关联。因此，λ5蛋白的表达先于完整μ重链蛋白的表达。这表明在μ-前B细胞中存在涉及Ig轻链样蛋白λ5和p16的发育调控蛋白复合物；在μ+前B细胞中存在λ5、p16和Ig重链蛋白，在B细胞和浆细胞中存在Ig重链和传统的Ig轻链蛋白。
