Unité de Rétrovirologie, Centre International de Recherches Médicales de Franceville, Franceville, Gabon.
Retrovirology. 2010 Dec 14;7:105. doi: 10.1186/1742-4690-7-105.
Each of the pathogenic human retroviruses (HIV-1/2 and HTLV-1) has a nonhuman primate counterpart, and the presence of these retroviruses in humans results from interspecies transmission. The passage of another simian retrovirus, simian foamy virus (SFV), from apes or monkeys to humans has been reported. Mandrillus sphinx, a monkey species living in central Africa, is naturally infected with SFV. We evaluated the natural history of the virus in a free-ranging colony of mandrills and investigated possible transmission of mandrill SFV to humans.
We studied 84 semi-free-ranging captive mandrills at the Primate Centre of the Centre International de Recherches Médicales de Franceville (Gabon) and 15 wild mandrills caught in various areas of the country. The presence of SFV was also evaluated in 20 people who worked closely with mandrills and other nonhuman primates. SFV infection was determined by specific serological (Western blot) and molecular (nested PCR of the integrase region in the polymerase gene) assays. Seropositivity for SFV was found in 70/84 (83%) captive and 9/15 (60%) wild-caught mandrills and in 2/20 (10%) humans. The 425-bp SFV integrase fragment was detected in peripheral blood DNA from 53 captive and 8 wild-caught mandrills and in two personnel. Sequence and phylogenetic studies demonstrated the presence of two distinct strains of mandrill SFV, one clade including SFVs from mandrills living in the northern part of Gabon and the second consisting of SFV from animals living in the south. One man who had been bitten 10 years earlier by a mandrill and another bitten 22 years earlier by a macaque were found to be SFV infected, both at the Primate Centre. The second man had a sequence close to SFVmac sequences. Comparative sequence analysis of the virus from the first man and from the mandrill showed nearly identical sequences, indicating genetic stability of SFV over time.
Our results show a high prevalence of SFV infection in a semi-free-ranging colony of mandrills, with the presence of two different strains. We also showed transmission of SFV from a mandrill and a macaque to humans.
每种致病性人类逆转录病毒（HIV-1/2 和 HTLV-1）都有一个非人类灵长类动物的对应物，这些逆转录病毒在人类中的存在是由于种间传播。另一种猿猴逆转录病毒，猿猴泡沫病毒（SFV），已从猿或猴传播到人类。生活在中非的 mandrillus sphinx 猴子自然感染 SFV。我们评估了自由放养的 mandrill 群体中病毒的自然史，并调查了 mandrill SFV 向人类传播的可能性。
我们在加蓬国际医学研究中心的灵长类动物中心（加蓬）研究了 84 只半自由放养的圈养 mandrills 和在该国不同地区捕获的 15 只野生 mandrills。还评估了 20 名与 mandrills 和其他非人类灵长类动物密切接触的人的 SFV 感染情况。SFV 感染通过特异性血清学（Western blot）和分子学（聚合酶基因中整合酶区域的巢式 PCR）检测确定。在 84 只圈养和 15 只野生捕获的 mandrills 中发现 70/84（83%）和 9/15（60%）血清阳性，在 20 名人类中发现 2/20（10%）血清阳性。在 53 只圈养和 8 只野生捕获的 mandrills 和两名人员的外周血 DNA 中检测到 425bp SFV 整合酶片段。序列和系统发育研究表明，存在两种不同的 mandrill SFV 株，一个分支包括来自加蓬北部地区的 mandrills 的 SFV，另一个分支包括来自南部地区的动物的 SFV。10 年前被一只 mandrill 咬伤的一名男子和 22 年前被一只猕猴咬伤的另一名男子被发现感染了 SFV，均在灵长类动物中心。第二个人的序列与 SFVmac 序列接近。对来自第一个男人和 mandrill 的病毒的比较序列分析表明，SFV 随时间具有遗传稳定性。
我们的结果表明，在半自由放养的 mandrill 群体中，SFV 感染率很高，存在两种不同的株。我们还表明 SFV 从 mandrill 和猕猴传播给人类。
