Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA.
Anal Biochem. 2011 Apr 15;411(2):292-6. doi: 10.1016/j.ab.2010.12.014. Epub 2010 Dec 13.
The present work discusses an alternative procedure to obtain static light scattering (SLS) parameters in a dilute and semidilute concentration regime from a dynamic light scattering (DLS) instrument that uses an avalanche photodiode (APD) for recording the scattered intensity signal. An APD enables one to perform both SLS and DLS measurements by photon counting and photon correlation, respectively. However, due to the associated recovery time, the APDs are susceptible to saturation (above 1000 kcps), which may limit the measurements in systems that scatter too much light. We propose an alternative way of obtaining the SLS parameters with instruments that use APD for recording signal intensities.
本工作讨论了一种从使用雪崩光电二极管 (APD) 记录散射强度信号的动态光散射 (DLS) 仪器中在稀相和半稀相浓度范围内获得静态光散射 (SLS) 参数的替代方法。APD 可分别通过光子计数和光子相关来执行 SLS 和 DLS 测量。然而，由于相关的恢复时间，APD 容易饱和（高于 1000 kcps），这可能会限制在散射光过多的系统中的测量。我们提出了一种使用 APD 记录信号强度的仪器来获取 SLS 参数的替代方法。
