Karalova E M, Bakhshinian M Z, Magakian Iu A
Tsitologiia. 1990;32(1):47-53.
It has been found that nearly 50% of the lymph node and spleen macrophages (MP) of the CBA line mice contain DNA at levels superior to the diploid value (H2c--H4c in mononuclear MP, and up to H16c among polynuclear ones, the latter comprising 2.5-9.0% of the whole MP population). No DNA synthesis and mitosis were detected by autoradiography, cytophotometry, and cytomorphological analysis. During carcinogenesis the proportion of MP with elevated DNA amounts ("activated MPs") decreases due to their migration to tumours. Also immature MPs (1.6%) appear in the population, which synthesize DNA, but do not divide. Injection of retinoids restores the percentage of MPs with elevated DNA amounts to the levels characteristic of the intact animals, the fraction of DNA-synthetizing cells increasing up to 2.8%. It is proposed that retinoids may accelerate the processes of MP maturation, activation and renewing. A mechanism of cooperative action of MPs and retinoids is discussed in addition to the role of DNA hyper-replication.
已发现，CBA品系小鼠近50%的淋巴结和脾脏巨噬细胞（MP）所含DNA水平高于二倍体值（单核MP中为H2c - H4c，多核MP中高达H16c，后者占整个MP群体的2.5 - 9.0%）。通过放射自显影、细胞光度测定法和细胞形态学分析未检测到DNA合成和有丝分裂。在致癌过程中，DNA含量升高的MP（“活化MP”）比例因它们向肿瘤迁移而降低。此外，群体中还出现了未成熟的MP（1.6%），它们合成DNA但不分裂。注射类视黄醇可将DNA含量升高的MP百分比恢复到完整动物的特征水平，DNA合成细胞的比例增加至2.8%。有人提出，类视黄醇可能会加速MP成熟、活化和更新的过程。除了DNA超复制的作用外，还讨论了MP与类视黄醇协同作用的机制。
