[Rb1和Rg1对肾缺血/再灌注血清诱导HK-2细胞凋亡中Bcl-2、Bax表达的影响]
[Effects of Rb1 and Rg1 on the expression of Bcl-2, Bax in apoptosis of HK-2 cells induced by the serum of kidney ischemia/reperfusion].
作者信息
Zhu Min-Xia, Ran Bing, Feng Zhi-Qiang, Pan Qiang-Wen
机构信息
Medical Department of Tibet National College, Xianyang 712082, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Nov;25(4):496-9.
AIM
To explore effects of Ginsenosides (Rb1, Rg1) on the expression of Bcl-2, Bax in the serum of kidney ischemia/reperfusion inducing apoptosis of HK-2 cells.
METHODS
The serum of rabbits with renal ischemia/reperfusion (SIR) and the control serum of rabbits (SC) were acquired and cultured with HK-2 cells. Detected apoptosis with TUNEL assay. The experiment was designed as: control group,ischemia/ reperfusion group, Rb1 blocking group and Rg1 blocking group. To detect the expression of Bcl-2, Bax with immunocytochemistry after 24 hours' cultured.
RESULTS
The expression of Bax in Rb1 blocking group and Rg1 blocking group were significantly decreased (P < 0.01), the ratios of Bcl-2/Bax were increased as compared with ischemia/reperfusion group.
CONCLUSION
Rb1 and Rg1 have protective effects on apoptosis of HK-2 cells induced by serum of kidney ischemia/reperfusion.
目的
探讨人参皂苷（Rb1、Rg1）对肾缺血/再灌注诱导HK-2细胞凋亡血清中Bcl-2、Bax表达的影响。
方法
获取肾缺血/再灌注兔血清（SIR）和对照兔血清（SC），并与HK-2细胞共同培养。采用TUNEL法检测细胞凋亡情况。实验分组为：对照组、缺血/再灌注组、Rb1阻断组和Rg1阻断组。培养24小时后，采用免疫细胞化学法检测Bcl-2、Bax的表达。
结果
Rb1阻断组和Rg1阻断组中Bax的表达显著降低（P < 0.01），与缺血/再灌注组相比，Bcl-2/Bax比值升高。
结论
Rb1和Rg1对肾缺血/再灌注血清诱导的HK-2细胞凋亡具有保护作用。
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