Department of Orthopedic, China-Japan Union Hospital, Jilin University, No.126 Xiantai Street, Changchun 130033, China.
Department of Nephrology, The fourth hospital of Jilin University, No.2643 Dongfeng Avenue, Changchun, Jilin Province 130033, China.
Exp Mol Pathol. 2018 Dec;105(3):229-235. doi: 10.1016/j.yexmp.2018.09.001. Epub 2018 Sep 2.
The aim of this study was to confirm the effects of ginsenoside Rb1 on neural cell apoptosis in the spinal cord of rats with spinal cord ischemia-reperfusion injury (SCII) and to explore its potential mechanisms. A total of 100 healthy adult Sprague-Dawley (SD) rats were randomly divided into four groups: normal control (n = 10), sham-operated (n = 10), SCII model (n = 40), and ginsenoside Rb1-treated groups (n = 40). Basso, Beattie, Bresnahan (BBB) scale was used to examine rat hindlimb locomotor function. Nissl and Tunnel staining were used to observe neural cell injury and apoptosis, respectively, in the spinal cord of rats with SCII. Immunofluorescence staining was performed to detect the expression of Bax and Bcl-2. The levels of caspase-3 and phosphorylated Ask-1 (p-Ask-1) were detected by western blotting. Ginsenoside Rb1 prevented neural cell apoptosis in the spinal cord and improved hindlimb locomotor dysfunction of rats (P < .05). Moreover, SCII-induced upregulation of caspase-3 and p-Ask-1 levels and the Bax/Bcl-2 ratio were significantly decreased by ginsenoside Rb1 (P < .05). The protective effects of ginsenoside Rb1 on neural cells in the spinal cord of rats with SCII were mediated by the ginsenoside Rb1-induced downregulation of caspase-3 and p-Ask-1 levels and the Bax/Bcl-2 ratio.
本研究旨在证实人参皂苷 Rb1 对脊髓缺血再灌注损伤（SCII）大鼠脊髓神经细胞凋亡的影响，并探讨其潜在机制。将 100 只健康成年 SD 大鼠随机分为四组：正常对照组（n=10）、假手术组（n=10）、SCII 模型组（n=40）和人参皂苷 Rb1 治疗组（n=40）。采用 Basso、Beattie、Bresnahan（BBB）评分法评估大鼠后肢运动功能。尼氏染色和Tunnel 染色分别观察 SCII 大鼠脊髓神经细胞损伤和凋亡情况，免疫荧光染色检测 Bax 和 Bcl-2 的表达，Western blot 检测 caspase-3 和磷酸化 Ask-1（p-Ask-1）的水平。人参皂苷 Rb1 可预防 SCII 大鼠脊髓神经细胞凋亡，改善大鼠后肢运动功能障碍（P<0.05）。此外，人参皂苷 Rb1 可显著下调 caspase-3 和 p-Ask-1 水平及 Bax/Bcl-2 比值，从而抑制 SCII 诱导的上述变化（P<0.05）。人参皂苷 Rb1 对 SCII 大鼠脊髓神经细胞的保护作用是通过下调 caspase-3 和 p-Ask-1 水平及 Bax/Bcl-2 比值介导的。
