Lu Chang-Zhu, Shi Yan-Hui, Jiao Run-Sheng, Kong Ya-Kun, Yao Lan-Chun
Department of Physiology, Qiqihar Medical College, Qiqihar 161006, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Nov;25(4):565-8.
To observe the effect of vitamin E (VE) on ovarian apoptosis-related protein Bcl-2 and Bax and its impact on antioxidant capacity in aged female rats and to study the senility-delaying effect and mechanism of VE on ovary.
Natural aging female rats were given different doses of exogenous VE. Then apoptosis regulatory protein Bcl-2, Bax expression in ovarian grandlose cells were detected by using immunohistochemical methods and Western blot. The contents of serum total superoxide dismutase (SOD) activity and malondialdehyde (MDA) were detected by using biochemical methods.
Contrasted with adult control group, the level of Bcl-2 expression in Senile control group was lower and the level of Bax expression was higher (P < 0.01), Serum SOD activity decreased and the level of MDA significantly increased (P < 0.01). Contrasted with senile control group, the level of Bcl-2 expression increased in VE group, the level of Bax expression decreased (P < 0.05), the level of MDA expression significantly decreased (P < 0.01).
VE can regulate apoptosis-related protein Bcl-2, Bax expression and confront free radical damage which contribute to a protective effect for ovarian grandiose cells.
观察维生素E（VE）对衰老雌性大鼠卵巢凋亡相关蛋白Bcl-2和Bax的影响及其对抗氧化能力的作用，探讨VE对卵巢的延缓衰老作用及机制。
给予自然衰老雌性大鼠不同剂量的外源性VE。采用免疫组织化学方法和蛋白质印迹法检测卵巢颗粒细胞中凋亡调节蛋白Bcl-2、Bax的表达。采用生化方法检测血清总超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。
与成年对照组相比，老年对照组Bcl-2表达水平降低，Bax表达水平升高（P<0.01），血清SOD活性降低，MDA水平显著升高（P<0.01）。与老年对照组相比，VE组Bcl-2表达水平升高，Bax表达水平降低（P<0.05），MDA表达水平显著降低（P<0.01）。
VE可调节凋亡相关蛋白Bcl-2、Bax的表达，对抗自由基损伤，对卵巢颗粒细胞具有保护作用。
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