[东莨菪碱对小鼠吗啡依赖的影响及其作用与海马细胞内钙的关系]
[Effects of scopolamine on morphine-dependence in mice and the relationship between the effect and hippocampus intracellular calcium].
作者信息
Wang Li-guang, Guo Xin-hua, Liu Ling-Yun, Peng Bai-ying, Liu Yu-ning, Qiao Jun-hong
机构信息
Department of Physiology, Institute of Basic Medicine, HeBei Engineering University, Handan 056002, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 Aug;22(3):307-9.
AIM
To observe the effect and mechanism of scopolamine on morphine(Mor)-induced mice dependence.
METHODS
The Mor-dependent mice model was established by intraperitoneal (ip) administered Mor for seven days. Pain threshold, times of jump and hippocampus intracellular free calcium ion concentration ([Ca2+]i) were determined by the heat plate test, naloxone (Nal)-precipitated jumping response and flow cytometry, respectively.
RESULTS
The pain threshold of Mor-dependent mice decreased significantly while there was a marked increase in times of jump, the rate of jumping animals and hippocampus [Ca2+]i. Co-administered scopolamine, the pain threshold of Mor-dependent mice increased significantly; the number of jump, the rate of jumping animals and hippocampus [Ca2+]i all decreased significantly.
CONCLUSION
Scopolamine could antagonize the Mor-induced mice dependence, which could be related to decreasing the levels of brain intracellular free calcium.
目的
观察东莨菪碱对吗啡诱导的小鼠依赖性的影响及其机制。
方法
通过腹腔注射吗啡7天建立吗啡依赖小鼠模型。分别采用热板法、纳洛酮催促跳跃反应及流式细胞术测定痛阈、跳跃次数及海马细胞内游离钙离子浓度（[Ca2+]i）。
结果
吗啡依赖小鼠痛阈显著降低，跳跃次数、跳跃动物率及海马[Ca2+]i显著升高。联合给予东莨菪碱后，吗啡依赖小鼠痛阈显著升高；跳跃次数、跳跃动物率及海马[Ca2+]i均显著降低。
结论
东莨菪碱可拮抗吗啡诱导的小鼠依赖性，其机制可能与降低脑内细胞内游离钙离子水平有关。
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