[下丘脑室旁核区域谷氨酸受体拮抗对清醒大鼠压力感受性反射的影响]
[Effect of antagonism of glutamate receptors in the PVN region on baroreflex in conscious rats].
作者信息
Cui Gui-yu, Yin Gui-dong, Jiang Hai-ying, Jin Yuan-zhe, Jin Qing-hua
机构信息
Department of Physiology and Pathophysiology, Yanbian University Medical College, Yanji 133000, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Nov;24(4):421-5.
AIM
To investigate the possible involvement of glutamate(Glu) in the paraventricular nucleus (PVN) in the central regulation of baroreflex.
METHODS
The baroreflex was induced by intravenous injection of phenylephrine in conscious rats, and the extracellular concentration of Glu in the PVN region was measured by microdialysis and high performance liquid chromatography (HPLC) techniques. To determine whether the observed Glu release was involved in the baroreflex, NMDA and non-NMDA receptor antagonists, MK-801 and CNQX, were perfused in the PVN region during baroreflex.
RESULTS
During baroreflex, the Glu concentration in the PVN region immediately increased to 384.82% +/- 91.77% of basal level (P < 0.01). (2) During baroreflex, direct perfusion of MK-801 and CNQX in the PVN were attenuated the increase of blood pressure and enhanced the decrease of HR (P < 0.01),resulting a significant increase in baroreflex sensitivity (P < 0.01).
CONCLUSION
Glutamate in PVN is involved in central regulation of baroreflex, which may inhibit baroreflex via ionothopic glutamate receptors.
目的
探讨谷氨酸（Glu）在室旁核（PVN）中参与压力感受性反射中枢调节的可能性。
方法
在清醒大鼠中通过静脉注射去氧肾上腺素诱导压力感受性反射，采用微透析和高效液相色谱（HPLC）技术测量PVN区域细胞外Glu浓度。为确定观察到的Glu释放是否参与压力感受性反射，在压力感受性反射期间将NMDA和非NMDA受体拮抗剂MK-801和CNQX灌注到PVN区域。
结果
（1）在压力感受性反射期间，PVN区域的Glu浓度立即增加至基础水平的384.82%±91.77%（P<0.01）。（2）在压力感受性反射期间，在PVN中直接灌注MK-801和CNQX可减弱血压升高并增强心率降低（P<0.01），导致压力感受性反射敏感性显著增加（P<0.01）。
结论
PVN中的谷氨酸参与压力感受性反射的中枢调节，可能通过离子型谷氨酸受体抑制压力感受性反射。
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