[克隆美国王鸽γ-氨基丁酸A受体α1、β2和γ2亚基的编码cDNA序列]
[Cloning the coding cDNA sequence of alpha1, beta2 and gamma2 subunit of GABA-A receptor in American king pigeon].
作者信息
Cheng Guang-dong, Cui Ya-li, Xu Shi-wen, Li Shu
机构信息
College of Veterinary Medicine, Northeast Argricultural University, Harbin 150030, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2008 Nov;24(4):453-6.
AIM
To clone and analyse the coding cDNA sequence of alpha1, beta2 and gamma2 subunit of GABAA receptor in American king Pigeon.
METHODS
Withdrew total RNA from the American king pigeon brain, reverse transcribing general primers to acquire a gene set cDNA. Designing specific primers of three subunit mRNA of the GABAA receptor, by RT-PCR respectively expanded the conservative gene of al subunit, beta2 subunit and gamma2 subunit of GABAA receptor, and carried on clone, plastid identification and the sequence measurese of three genes.
RESULTS
The experiment on sequence measures has succeeded that sequence analysis indicated that lengths of the conservative gene of alpha1 subunit, beta2 subunit and gamma2 subunit of GABAA receptor was respectively 899 bp, 597 bp and 563 bp, homology on reference sequence was respectively 94.99%, 94.64% and 96.28%.
CONCLUSION
Homology is high on the conservative gene of alpha1 subunit, beta2 subunit and gamma2 subunit of GABAA receptor of brain tissue of pigeon and chicken but there is a discriminating characteristic in different kinds of animals.
目的
克隆并分析美国王鸽γ-氨基丁酸A型（GABAA）受体α1、β2和γ2亚基的编码cDNA序列。
方法
从美国王鸽脑组织中提取总RNA，用通用引物反转录获得基因集cDNA。设计GABAA受体三个亚基mRNA的特异性引物，通过逆转录聚合酶链反应（RT-PCR）分别扩增GABAA受体α1亚基、β2亚基和γ2亚基的保守基因，并对这三个基因进行克隆、质粒鉴定和序列测定。
结果
序列测定实验成功，序列分析表明GABAA受体α1亚基、β2亚基和γ2亚基保守基因的长度分别为899 bp、597 bp和563 bp，与参考序列的同源性分别为94.99%、94.64%和96.28%。
结论
鸽脑组织中GABAA受体α1亚基、β2亚基和γ2亚基的保守基因与鸡的同源性较高，但在不同种类动物中存在鉴别特征。
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