DNA损伤剂预处理对紫外线照射的盘基网柄菌细胞杀伤中热增强作用的影响。
Influence of pre-treatment with DNA-damaging agents on hyperthermic potentiation in cell killing of ultraviolet light-irradiated Dictyostelium discoideum.
作者信息
Iwata K, Ohishi H, Ohnishi T
机构信息
Department of Oncoradiology, Nara Medical University, Japan.
出版信息
Int J Hyperthermia. 1990 May-Jun;6(3):619-27. doi: 10.3109/02656739009140958.
Heat treatment at 30 degrees C for 15 min immediately after ultraviolet (UV) irradiation enhanced cell killing of Dictyostelium discoideum. However, when the cells were UV-irradiated and cultured at 23 degrees C for 30 min before giving UV irradiation and heat treatment, the enhancement of cell killing by heat treatment immediately after post-UV irradiation was depressed. Similar depressions in the heat enhancement of cell killing of UV-irradiated cells were also observed in the case of the cells cultured after pretreatment with 4-nitroquinoline 1-oxide, cis-platinum(II) diaminedichloride or 8-methoxypsoralen photoaddition, but not with N-methyl-N'-nitro-N-nitrosoguanidine or methylmethanesulphonate. These results suggest that the depression in heat enhancement in cell killing of UV-irradiated cells may be deeply related with kinds of DNA lesions or DNA repair processes for them.
紫外线（UV）照射后立即在30摄氏度下热处理15分钟可增强盘基网柄菌的细胞杀伤作用。然而，当细胞先在23摄氏度下进行紫外线照射并培养30分钟，然后再进行紫外线照射和热处理时，紫外线照射后立即进行热处理对细胞杀伤的增强作用会受到抑制。在用4-硝基喹啉1-氧化物、顺铂（II）二胺二氯化物或8-甲氧基补骨脂素光加成预处理后培养的细胞中，也观察到紫外线照射细胞的热增强细胞杀伤作用有类似的抑制，但在用N-甲基-N'-硝基-N-亚硝基胍或甲基磺酸甲酯预处理的细胞中未观察到。这些结果表明，紫外线照射细胞的热增强细胞杀伤作用的抑制可能与DNA损伤的种类或针对这些损伤的DNA修复过程密切相关。
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