Guangdong Institute of Microbiology, Guangzhou, China.
J Appl Microbiol. 2011 Feb;110(2):580-6. doi: 10.1111/j.1365-2672.2010.04913.x. Epub 2010 Dec 16.
To investigate the role of soluble and insoluble iron in azoreduction by resting cells of Shewanella decolorationis S12.
A series of analytical experiments were carried out. Results showed that insoluble Fe(2) O(3) all delayed the reduction of amaranth but did not inhibit it. Adsorption to Fe(2) O(3) particles by the bacterial cell surface could be the reason leading to the delay in azoreduction. For the soluble iron, an important finding was that azoreduction activities were inhibited by soluble iron in high concentration because of its higher redox potential, and the inhibition was strengthened when the electron donor supply was insufficient. However, activities of azoreduction could be enhanced by low concentration of soluble iron. This stimulating effect was because of the electron transfer but not the cell growth.
The effects of iron on azoreduction by the resting cells depended on the solubility and concentration of the iron compounds, which was different from what was observed by the growing cells in the previous studies.
This study has both theoretical significance in the microbial physiology and practical significance in the bioremediation of azo dyes-contaminated environment.
研究可溶性和不溶性铁在 Shewanella decolorationis S12 静止细胞偶氮还原中的作用。
进行了一系列分析实验。结果表明，不溶性 Fe(2)O(3)均延迟了苋菜红的还原，但没有抑制它。细菌细胞表面对 Fe(2)O(3)颗粒的吸附可能是导致偶氮还原延迟的原因。对于可溶性铁，一个重要的发现是，由于其较高的氧化还原电位，高浓度的可溶性铁抑制了偶氮还原活性，并且当电子供体供应不足时，抑制作用会增强。然而，低浓度的可溶性铁可以增强偶氮还原活性。这种刺激作用是由于电子转移而不是细胞生长。
铁对静止细胞偶氮还原的影响取决于铁化合物的溶解度和浓度，这与以前研究中观察到的生长细胞不同。
本研究在微生物生理学方面具有理论意义，在偶氮染料污染环境的生物修复方面具有实际意义。
