Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials (Liaoning University), Shenyang 110036, China.
Biosens Bioelectron. 2011 Jan 15;26(5):2632-7. doi: 10.1016/j.bios.2010.11.024. Epub 2010 Nov 25.
A novel polymeric ionic liquid functionalized graphene, poly(1-vinyl-3-butylimidazolium bromide)-graphene (denoted as poly(ViBuIm(+)Br(-))-G), was synthesized. FTIR, UV-vis spectra and TEM were used to characterize the formation of as synthesized nanocomposites. Due to the modification of the polymeric ionic liquid, poly(ViBuIm(+)Br(-))-G can not only be dispersed well in aqueous solutions to form a homogeneous colloidal suspension of individual nanosheets, but also exhibit a strong positive charge. Based on self-assembly, the negatively charged glucose oxidase (GOD) was immobilized onto the poly(ViBuIm(+)Br(-))-G to form a GOD/poly(ViBuIm(+)Br(-))-G/glassy carbon (GC) electrode under mild conditions. With the advantage of both poly(ViBuIm(+)Br(-)) and graphene, poly(ViBuIm(+)Br(-))-G can provide a favorable and conductive microenvironment for the immobilized GOD and thus promote their direct electron transfer at the GC electrode. Furthermore, the GOD/poly(ViBuIm(+)Br(-))-G/GC electrode displayed an excellent sensitivity, together with a wide linear range and excellent stability for the detection of glucose. Accordingly, these unique properties of such novel nanocomposite generate a promising platform for the construction of mediator-free enzymatic biosensors.
一种新型聚合离子液体功能化石墨烯，聚（1-乙烯基-3-丁基咪唑溴化盐）-石墨烯（表示为 poly(ViBuIm(+)Br(-))-G）被合成。FTIR、UV-vis 光谱和 TEM 用于表征合成纳米复合材料的形成。由于聚合离子液体的修饰，poly(ViBuIm(+)Br(-))-G 不仅可以很好地分散在水溶液中形成单个纳米片的均匀胶体悬浮液，而且还表现出很强的正电荷。基于自组装，带负电荷的葡萄糖氧化酶（GOD）被固定在 poly(ViBuIm(+)Br(-))-G 上，在温和的条件下形成 GOD/poly(ViBuIm(+)Br(-))-G/玻碳（GC）电极。由于 poly(ViBuIm(+)Br(-)) 和石墨烯的优势，poly(ViBuIm(+)Br(-))-G 可以为固定化的 GOD 提供有利的导电微环境，从而促进它们在 GC 电极上的直接电子转移。此外，GOD/poly(ViBuIm(+)Br(-))-G/GC 电极对葡萄糖的检测表现出优异的灵敏度、宽线性范围和良好的稳定性。因此，这种新型纳米复合材料的独特性质为构建无介体酶生物传感器提供了一个有前途的平台。
