Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Yufu, Japan. kawayas @ med.oita-u.ac.jp
Gynecol Obstet Invest. 2011;71(4):240-4. doi: 10.1159/000319615. Epub 2010 Dec 16.
Metallothionein (MT) is known to bind to metals with high affinity. The potential for MT-1 mRNA expression in endometrial stromal cells (ESC) and amniotic cells in response to cytokines and cadmium chloride (CdCl(2)) was evaluated.
Human ESC were cultured and treated with interleukin-1α, 12-O-tetradecanoylphobol 13-acetate (TPA), forskolin, transforming growth factor-β, and CdCl(2). Amnion-derived (WISH) cells were also cultured and treated with the same reagents. The levels of MT mRNA were evaluated by Northern blot analysis in ESC and WISH cells.
In response to treatment with CdCl(2) (0.01-10 μM), the expression of MT mRNA markedly increased in ESC and WISH cells in a dose-dependent manner. On the other hand, the expression of MT mRNA did not increase after treatment with interleukin-1α (1 nM), TPA (10 nM), forskolin (1 μM) or transforming growth factor-β (1 nM) in these cells.
These findings indicate that MT expression in ESC and WISH cells is sensitive to the CdCl(2) concentration, which is known to be evaluated in cigarette smokers. The present results suggest that increased levels of MT may affect metal metabolism at the feto-maternal interface.
金属硫蛋白（MT）与金属具有高亲和力。评估了细胞因子和氯化镉（CdCl（2））对子宫内膜基质细胞（ESC）和羊膜细胞中 MT-1 mRNA 表达的潜在作用。
培养人 ESC 并分别用白细胞介素-1α、12-O-十四烷酰佛波醇 13-乙酸酯（TPA）、佛司可林、转化生长因子-β和 CdCl（2）处理。还培养了羊膜衍生的（WISH）细胞并使用相同的试剂进行处理。通过 Northern blot 分析在 ESC 和 WISH 细胞中评估 MT mRNA 的水平。
用 CdCl（2）（0.01-10 μM）处理后，ESC 和 WISH 细胞中 MT mRNA 的表达呈剂量依赖性显著增加。另一方面，这些细胞用白细胞介素-1α（1 nM）、TPA（10 nM）、佛司可林（1 μM）或转化生长因子-β（1 nM）处理后，MT mRNA 的表达没有增加。
这些发现表明 MT 在 ESC 和 WISH 细胞中的表达对 CdCl（2）浓度敏感，而 CdCl（2）浓度已知可用于评估吸烟人群。本研究结果表明，金属硫蛋白水平的升高可能会影响胎-母界面的金属代谢。
