Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea.
Gynecol Obstet Invest. 2011;72(1):32-6. doi: 10.1159/000322222. Epub 2010 Dec 15.
To investigate the dose-dependent effect of sphingosine-1-phosphate (S1P) supplementation on meiotic maturation, apoptosis and developmental competence of immature mouse oocytes.
Immature oocytes from 5- to 6-week-old BDF1 mice were incubated in maturation medium containing S1P with six different doses (10 nM, 50 nM, 100 nM, 500 nM, 1 μM, and 2 μM) for 18-19 h. Fertilization and blastocyst formation was assessed. All blastocysts were stained by anti-caspase antibody.
Treatment with 100 nM, 500 nM and 1 μM S1P showed significantly higher blastulation rate [66.0, 62.1 and 66.2%, respectively, p < 0.05 when compared with control (40.0%)]. Blastomere count was similar across six treatment groups, but the percentage of apoptotic blastomere was significantly lower in the 100 nM, 500 nM, and 2 μM S1P-treated groups [5.4, 6.5, and 3.7%, respectively, p < 0.05 when compared with control (10.6%)].
Considering the blastulation rate together with the apoptotic event in the blastocysts, adding 100 and 500 nM S1P within oocyte maturation medium could be beneficial in the development of immature mouse oocytes.
研究鞘氨醇-1-磷酸（S1P）补充对未成熟小鼠卵母细胞减数分裂成熟、凋亡和发育能力的剂量依赖性影响。
将来自 5-6 周龄 BDF1 小鼠的未成熟卵母细胞在含有 S1P 的成熟培养基中孵育 18-19 小时，S1P 有六个不同剂量（10 nM、50 nM、100 nM、500 nM、1 μM 和 2 μM）。评估受精和囊胚形成情况。所有囊胚均用抗半胱天冬酶抗体染色。
用 100 nM、500 nM 和 1 μM S1P 处理，囊胚孵化率显著升高[分别为 66.0%、62.1%和 66.2%，与对照组（40.0%）相比，p<0.05]。六个处理组的卵裂球计数相似，但在 100 nM、500 nM 和 2 μM S1P 处理组中，凋亡卵裂球的百分比显著降低[分别为 5.4%、6.5%和 3.7%，与对照组（10.6%）相比，p<0.05]。
考虑到囊胚孵化率和囊胚中的凋亡事件，在卵母细胞成熟培养基中添加 100 和 500 nM S1P 可能有利于未成熟小鼠卵母细胞的发育。
