霉酚酸酯加阿霉素对HepG-2细胞的作用。
Effect of mycophenolate mofetil plus adriamycin on HepG-2 cells.
作者信息
Chu Yan-Kui, Liu Yi, Yin Ji-Kai, Wang Nan, Cai Liang, Lu Jian-Guo
机构信息
Yan-Kui Chu, Yi Liu, Ji-Kai Yin, Nan Wang, Liang Cai, Jian-Guo Lu, Department of General Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi Province, China.
出版信息
World J Hepatol. 2010 Aug 27;2(8):311-7. doi: 10.4254/wjh.v2.i8.311.
AIM
To investigate the influence of mycophenolate mofetil (MMF) plus adriamycin (ADM) on hepatocellular carcinoma (HCC) cells.
METHODS
HCC cells were treated with 100 μg/ml of MMF alone (MMF group), 1 μg/mL of adriamycin (ADM group) alone, or a combination of the drugs (MMF + ADM group). We performed an 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay to measure the growth inhibition rate of HCC cells. Flow cytometry was used to determine the percentage of cells in different phases of the cell cycle and the number of apoptotic cells. Hoechst 33258 staining revealed the morphological changes associated with apoptosis in HCC cells.
RESULTS
The results of MTT assays revealed that monotherapy with ADM or MMF showed inhibition of cell growth, while MMF + ADM therapy afforded an inhibition rate of more than 90% with cell distribution in G1 and G2/M phase greater than that in S phase. MMF + ADM treatment also downregulated Bcl-2 expression markedly. The growth of HCC cells was markedly inhibited and apoptosis was enhanced in all the 3 groups. Compared with other 2 groups, the MMF + ADM group showed more obvious apoptosis of cells.
CONCLUSION
The MMF plus ADM combination exerts remarkable inhibitory effects on the growth of HCC cells.
目的
探讨霉酚酸酯（MMF）联合阿霉素（ADM）对肝癌（HCC）细胞的影响。
方法
将肝癌细胞分别用100μg/ml的MMF单独处理（MMF组）、1μg/mL的阿霉素单独处理（ADM组）或两种药物联合处理（MMF + ADM组）。我们进行了3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）试验以测量肝癌细胞的生长抑制率。采用流式细胞术测定细胞周期不同阶段的细胞百分比和凋亡细胞数量。Hoechst 33258染色揭示了肝癌细胞中与凋亡相关的形态学变化。
结果
MTT试验结果显示，ADM或MMF单药治疗均显示出对细胞生长的抑制作用，而MMF + ADM治疗的抑制率超过90%，G1期和G2/M期的细胞分布多于S期。MMF + ADM治疗还显著下调了Bcl-2表达。三组均显著抑制了肝癌细胞的生长并增强了凋亡。与其他两组相比，MMF + ADM组细胞凋亡更明显。
结论
MMF联合ADM对肝癌细胞的生长具有显著的抑制作用。
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