低密度脂蛋白脂质对IIA组分泌型磷脂酶A2活性的影响。

Effects of LDL lipids on activity of group IIA secretory phospholipase A2.

作者信息

Samoilova E V, Pirkova A A, Prokazova N V, Korotaeva A A

机构信息

Russian Cardiology Research-and-Production Complex, Federal Agency for Health Care and Social Development, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2010 Dec;150(1):39-41. doi: 10.1007/s10517-010-1062-5.

DOI:10.1007/s10517-010-1062-5

PMID:21161046

Abstract

Effects of phosphatidylcholine, oxidized phosphatidylcholine, sphyngomyelin, cholesterol, and cholesterol esters incorporated in LDL on activity of group IIA secretory phospholipase A2 from human cardiac myxoma were studied. Liposomes containing radioisotope-labeled phosphatidylethanolamine served as the substrate for group IIA secretory phospholipase A2. Oxidized phosphatidylcholine significantly stimulated activity of group IIA secretory phospholipase A2, while phosphatidylcholine in the same concentrations did not modify enzyme activity. Sphyngomyelin incorporated in LDL inhibited group IIA secretory phospholipase A2 activity. Cholesterol and cholesterol esters virtually did not modify enzyme activity. The results indicate that LDL phospholipids and their oxidized forms can be involved in regulation of group IIA secretory phospholipase A2. Study of the mechanisms regulating the proinflammatory group IIA secretory phospholipase A2 can promote the development of new approaches to the diagnosis and treatment of inflammatory processes.

摘要

研究了低密度脂蛋白（LDL）中所含的磷脂酰胆碱、氧化磷脂酰胆碱、鞘磷脂、胆固醇及胆固醇酯对人心脏黏液瘤中IIA组分泌型磷脂酶A2活性的影响。含有放射性同位素标记磷脂酰乙醇胺的脂质体用作IIA组分泌型磷脂酶A2的底物。氧化磷脂酰胆碱显著刺激IIA组分泌型磷脂酶A2的活性，而相同浓度的磷脂酰胆碱则不改变酶活性。LDL中所含的鞘磷脂抑制IIA组分泌型磷脂酶A2的活性。胆固醇及胆固醇酯几乎不改变酶活性。结果表明，LDL磷脂及其氧化形式可能参与IIA组分泌型磷脂酶A2的调节。对促炎的IIA组分泌型磷脂酶A2调节机制的研究有助于开发炎症性疾病诊断和治疗的新方法。