母乳喂养婴儿及其母亲肠道微生物群中双歧杆菌种群的物种和菌株变异性的分子遗传学研究。

Molecular genetic study of species and strain variability in bifidobacteria population in intestinal microflora of breast-fed infants and their mothers.

作者信息

Kulagina E V, Shkoporov A N, Kafarskaia L I, Khokhlova E V, Volodin N N, Donskikh E E, Korshunova O V, Efimov B A

机构信息

Russian State Medical University, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2010 Dec;150(1):61-4. doi: 10.1007/s10517-010-1069-y.

DOI:10.1007/s10517-010-1069-y

PMID:21161053

Abstract

Qualitative and quantitative composition of enteric bifidoflora was studied in a group of 13 mother-infant pairs. Pure cultures of Bifidobacterium strains were isolated from feces and their species were identified by PCR with species-specific primers or by partial sequencing of 16S rDNA. The strains were compared by REP-PCR. The most incident Bifidobacterium species in mothers were B. longum and B. adolescentis. The infants were mainly colonized by B. bifidum and B. longum. The mother and her baby were colonized by the same Bifidobacterium species in 9 of 13 cases. In 5 (38.5%) of these cases, these pairs of strains were identical by their REP-PCR profiles. These strains belonged to B. longum in one case, B. bifidum in 3 cases, and B. adolescentis in 1 case. Our results support the hypothesis on early colonization of infants with maternal bifidobacterium strains.

摘要

在一组13对母婴中研究了肠道双歧杆菌的定性和定量组成。从粪便中分离出双歧杆菌菌株的纯培养物，并通过使用种特异性引物的PCR或16S rDNA的部分测序来鉴定其种类。通过REP-PCR对菌株进行比较。母亲中最常见的双歧杆菌种类是长双歧杆菌和青春双歧杆菌。婴儿主要由双歧双歧杆菌和长双歧杆菌定植。在13例中的9例中，母亲和她的婴儿被相同的双歧杆菌种类定植。在其中5例（38.5%）中，这些菌株对通过其REP-PCR图谱是相同的。这些菌株在1例中属于长双歧杆菌，3例中属于双歧双歧杆菌，1例中属于青春双歧杆菌。我们的结果支持婴儿由母体双歧杆菌菌株早期定植的假说。