从哺乳动物肝脏、血清和胆汁中分离出的生物调节剂对蝾螈肝脏组织器官型培养状态的影响。

Effect of bioregulators isolated from the liver, blood serum, and bile of mammals on the state of newt liver tissue in organotypic culture.

作者信息

Yamskova V P, Borisenko A V, Krasnov M S, Il'ina A P, Rybakova E Yu, Malcev D I, Yamskov I A

机构信息

N. K. Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences; A. N. Nesmeyanov Institute of Elementorganic Compounds, Russian Academy of Sciences, Moscow, Russia.

出版信息

Bull Exp Biol Med. 2010 Dec;150(1):140-8. doi: 10.1007/s10517-010-1089-7.

DOI:10.1007/s10517-010-1089-7

PMID:21161073

Abstract

We developed models of in vitro organotypic culturing of newt liver tissue with and without adhesion to the substrate. The effects of bioregulators isolated from mammalian liver, blood serum, and bile were studied on the developed models and their specificity was demonstrated. The state of the liver was evaluated by the area of clusters of pigmented cells and by the number of mitoses in the connective tissue cells of the cortical layer. These bioregulators exhibited their biological effects only under conditions of roller organotypic culturing of newt liver tissue.

摘要

我们开发了蝾螈肝脏组织体外器官型培养模型，包括有和没有与底物黏附的情况。研究了从哺乳动物肝脏、血清和胆汁中分离出的生物调节剂对所开发模型的影响，并证明了它们的特异性。通过色素细胞簇的面积以及皮质层结缔组织细胞中的有丝分裂数来评估肝脏状态。这些生物调节剂仅在蝾螈肝脏组织的旋转器官型培养条件下才表现出其生物学效应。