Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, 21941-590, Rio de Janeiro, Rio de Janeiro, Brazil.
Parasitol Res. 2011 Jun;108(6):1473-8. doi: 10.1007/s00436-010-2194-2. Epub 2010 Dec 14.
We investigated the effects of platelet-activating factor (PAF) on the interaction of Trypanosoma cruzi with Rhodnius prolixus. The parasites (epimastigotes) were treated with PAF and/or WEB 2086 (PAF antagonist) for 1 h prior to the interaction experiments. PAF stimulated both in vivo and ex vivo interactions between T. cruzi and R. prolixus while WEB 2086 abrogated these effects. PAF-treated epimastigotes also showed an increase in surface negativity and in the amount of surface sialic acid. Neither of these effects was observed when the epimastigotes were treated with neuraminidase following PAF treatment. In the ex vivo interaction experiments, the number of epimastigotes bound to the midguts of the insects was reduced when the epimastigotes had been treated with neuraminidase. We conclude that PAF modulates the interaction of T. cruzi with R. prolixus by altering the amount of sialyl residues at the surface of the parasite.
我们研究了血小板激活因子 (PAF) 对克氏锥虫与丽蝇相互作用的影响。在相互作用实验之前，用 PAF 和/或 WEB 2086（PAF 拮抗剂）处理寄生虫（滋养体） 1 小时。PAF 刺激 T. cruzi 和 R. prolixus 之间的体内和体外相互作用，而 WEB 2086 则消除了这些作用。用 PAF 处理的滋养体的表面负电性和表面唾液酸含量也增加。当在用 PAF 处理后用神经氨酸酶处理滋养体时，没有观察到这些作用。在体外相互作用实验中，当用神经氨酸酶处理滋养体时，与昆虫中肠结合的滋养体数量减少。我们得出结论，PAF 通过改变寄生虫表面唾液酸残基的数量来调节 T. cruzi 与 R. prolixus 的相互作用。
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