Rodrigues C O, Dutra P M, Souto-Padrón T, Cordeiro R S, Lopes A H
Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Brazil.
Biochem Biophys Res Commun. 1996 Jun 25;223(3):735-40. doi: 10.1006/bbrc.1996.0965.
The effects of platelet-activating factor (PAF), at (10)-6M and (10)-9M, on cell growth and cell differentiation of Trypanosoma cruzi were investigated. Cell differentiation was evaluated by both light and electron microscopy. At the concentrations used, PAF slightly interfered with the protozoan growth. However, parasites growth in the presence of PAF were significantly more differentiated than those grown in the absence of PAF, beginning on the fourth day of culture. A specific PAF receptor antagonist (WEB 2086) totally abrogated PAF effect on cell differentiation. These findings indicate that PAF triggers the process of cell differentiation in T. cruzi and suggest that these parasites have receptors for PAF.
研究了浓度为10⁻⁶M和10⁻⁹M的血小板活化因子（PAF）对克氏锥虫细胞生长和细胞分化的影响。通过光学显微镜和电子显微镜评估细胞分化。在所使用的浓度下，PAF对原生动物的生长有轻微干扰。然而，从培养的第四天开始，在PAF存在下生长的寄生虫比在没有PAF的情况下生长的寄生虫分化程度明显更高。一种特异性PAF受体拮抗剂（WEB 2086）完全消除了PAF对细胞分化的影响。这些发现表明，PAF触发了克氏锥虫的细胞分化过程，并表明这些寄生虫具有PAF受体。
