人胚胎干细胞长期传代和分化后印迹基因的动态表达模式。
Dynamic expression patterns of imprinted genes in human embryonic stem cells following prolonged passaging and differentiation.
机构信息
Reproductive Medical Center, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan 2nd Avenue, Yuexiu, 510080, Guangzhou, China.
出版信息
J Assist Reprod Genet. 2011 Apr;28(4):315-23. doi: 10.1007/s10815-010-9524-2. Epub 2010 Dec 16.
PURPOSE
To evaluate the overall expression patterns of imprinted genes in human embryonic stem cells following long term culture and differentiation.
MATERIALS AND METHODS
Expression levels of 65 imprinted genes determined by PCR array were analyzed in one human embryonic stem cell line (cHES1) following prolonged passaging and differentiation.
RESULTS
Transcripts of 63 imprinted genes were detected in cHES1 cells. Expression levels of all but 5 imprinted genes did not correlate with passage numbers or differ in cells after passage 50 compared with those before passage 50. SLC22A2, SLC22A3, CPA, H19, COPG2IT1 and IGF2 expression were significantly increased in embryoid bodies compared with undifferentiated cells.
CONCLUSIONS
The global expression profiles of imprinted genes are generally stable in human embryonic stem cells after prolonged passaging and differentiation.
目的
评估人胚胎干细胞在长期培养和分化后印记基因的整体表达模式。
材料与方法
通过 PCR 阵列分析了一个人胚胎干细胞系（cHES1）在长时间传代和分化后的 65 个印记基因的表达水平。
结果
在 cHES1 细胞中检测到 63 个印记基因的转录本。除了 5 个印记基因外，所有印记基因的表达水平与传代数无关，并且在传代 50 次后的细胞与传代 50 次前的细胞相比没有差异。与未分化细胞相比，胚状体中 SLC22A2、SLC22A3、CPA、H19、COPG2IT1 和 IGF2 的表达显著增加。
结论
在长时间传代和分化后，人胚胎干细胞中印记基因的整体表达谱通常是稳定的。
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