Henan Normal University, Xinxiang 453007, People's Republic of China.
Mol Biol Rep. 2011 Oct;38(7):4423-8. doi: 10.1007/s11033-010-0570-8. Epub 2010 Dec 14.
It is important that endogenous reference genes for real-time RT-PCR be empirically evaluated for stability in different cell types, developmental stages, and/or sample treatment. To select the most stable endogenous reference genes during planarian regeneration, three housekeeping genes, 18S rRNA, ACTB and DjEF2, were identified and established expression levels by real-time RT-PCR. The data were analyzed by GeNorm and NormFinder software. Expression levels of the Djsix-1 gene were studied in parallel with ACTB and DjEF2 both or each and 18S rRNA as reference during regeneration. The results showed that ACTB was the most stable expressed reference gene in the planarian regeneration.
在不同的细胞类型、发育阶段和/或样本处理中，实时 RT-PCR 的内参基因的稳定性需要通过经验进行评估。为了在涡虫再生过程中选择最稳定的内参基因，我们通过实时 RT-PCR 鉴定并建立了三个管家基因 18S rRNA、ACTB 和 DjEF2 的表达水平。使用 GeNorm 和 NormFinder 软件对数据进行分析。在再生过程中，我们平行研究了 Djsix-1 基因与 ACTB 和 DjEF2 两者或每一个基因与 18S rRNA 作为参考的表达水平。结果表明，ACTB 是涡虫再生过程中表达最稳定的参考基因。
