Institute of Neurological Disease, West China Hospital, Sichuan University, Chengdu, China.
Cell Mol Neurobiol. 2011 Apr;31(3):407-14. doi: 10.1007/s10571-010-9633-6. Epub 2010 Dec 14.
Implanted neural stem cells (NSC) could improve neurological functions following spinal cord injury (SCI), but the optimal conditions for NSC transplantation are largely unknown, especially in transected spinal cord. This study investigated the effect and fate of NSC engrafted into spinal cords at different locations and time points following T(9) spinal cord transection. Engrafted NSC could survive and migrate in host spinal cords. Significant improvement in hindlimb locomotor functions associated with NSC survival was found in rats receiving NSC transplantation in the spinal cords rostral to the transection site at the subacute stage (7 days post operation), compared with those caudal to the transection site at the acute stage (at the time of injury). At 4 weeks post operation, CD68 immunohistochemical staining confirmed that macrophages were less in rostrally transplanted sites and in subacute groups than seen in caudal and acute transplanted rats. The present findings indicated that NSC transplantation into spinal cords rostral to transection site at the subacute stage is an optimal strategy for engrafted NSC survival and host behavioral improvement. It therefore would be available to the usage of NSC for the treatment of SCI in the future clinic trial.
植入的神经干细胞（NSC）可以改善脊髓损伤（SCI）后的神经功能，但 NSC 移植的最佳条件在很大程度上尚不清楚，特别是在脊髓横断部位。本研究探讨了 T（9）脊髓横断后不同部位和时间点移植 NSC 对脊髓的影响和命运。植入的 NSC 可以在宿主脊髓中存活和迁移。与急性阶段（损伤时）的尾部移植相比，在亚急性阶段（术后 7 天）于横断部位的脊髓前植入 NSC 的大鼠后肢运动功能明显改善，这与 NSC 存活有关。术后 4 周，CD68 免疫组化染色证实，与尾部和急性移植大鼠相比，在前部移植部位和亚急性组中，巨噬细胞较少。本研究结果表明，在亚急性阶段将 NSC 移植到横断部位的脊髓前部位是 NSC 存活和宿主行为改善的最佳策略。因此，它将可用于未来临床试验中 NSC 治疗 SCI。
