Laboratório de Neurociências and Instituto Nacional de Ciência e Tecnologia Translacional em Medicina (INCT-TM), Universidade do Extremo Sul Catarinense, Criciúma, SC, 88806-000, Brazil.
Neurochem Res. 2011 Mar;36(3):460-6. doi: 10.1007/s11064-010-0364-3. Epub 2010 Dec 16.
The present study was aimed to evaluate the behavioral and molecular effects of maternal deprivation in adult rats. To this aim, male rats deprived and non-deprived were assessed in the forced swimming and open-field tests in adult phase. In addition adrenocorticotrophin hormone (ACTH) levels was assessed in serum and brain-derived-neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and nerve growth factor (NGF) protein levels were assessed in prefrontal cortex, hippocampus and amygdala. We observed that maternal deprivation increased immobility time, and decreased climbing time, without affecting locomotor activity. ACTH circulating levels were increased in maternal deprived rats. Additionally, BDNF protein levels were reduced in the amygdala and NT-3 and NGF were reduced in both hippocampus and amygdala in maternal deprived rats, compared to control group. In conclusion, our results support the idea that behavioral, ACTH circulating levels and neurotrophins levels altered in maternal deprivation model could contribute to stress-related diseases, such as depression.
本研究旨在评估母体剥夺对成年大鼠行为和分子的影响。为此，在成年期，对被剥夺和未被剥夺的雄性大鼠进行强迫游泳和旷场试验评估。此外，还评估了血清中的促肾上腺皮质激素（ACTH）水平，以及前额叶皮质、海马体和杏仁核中的脑源性神经营养因子（BDNF）、神经生长因子-3（NT-3）和神经生长因子（NGF）蛋白水平。我们发现，母体剥夺增加了不动时间，减少了攀爬时间，而对运动活性没有影响。在被剥夺母爱的大鼠中，循环中的 ACTH 水平增加。此外，与对照组相比，在被剥夺母爱的大鼠中，杏仁核中的 BDNF 蛋白水平降低，而在海马体和杏仁核中，NT-3 和 NGF 水平降低。总之，我们的结果支持这样一种观点，即母体剥夺模型中行为、循环中的 ACTH 水平和神经营养因子水平的改变可能导致与应激相关的疾病，如抑郁症。
