表皮生长因子对台鸭（Anas platyrhynchos var. domestica）卵巢颗粒细胞孕酮产生的抑制作用。

Inhibitory effects of epidermal growth factor on progesterone production of ovarian granulosa cells in Tsaiya duck (Anas platyrhynchos var. domestica).

Department of Animal Science and Technology, Laboratory of Animal Physiology, National Taiwan University, Taipei, Taiwan, ROC.

Br Poult Sci. 2010 Dec;51(6):821-7. doi: 10.1080/00071668.2010.499141.

Abstract

The purpose of this study was to investigate the effects of epidermal growth factor (EGF) on progesterone (P(4)) secretion from ovarian granulosa cells in Tsaiya ducks (Anas platyrhynchos var. domestica). 2. We obtained the largest (F1) follicle from Tsaiya duck, the granulosa layer was separated and the cells were isolated according to their proximity to the germinal disc. 3. The granulosa cells were cultured in vitro, the culture media and the cells were used to determine P(4) and steroidogenic enzyme concentrations, respectively. 4. P(4) concentrations were decreased in cultured granulosa cells taken proximal to the germinal disc (GD) compared to those distal to the germinal disc (NGD). 5. EGF inhibited both basal and ovine luteinising hormone (oLH)-induced P(4) concentrations. It also inhibited the P(4) secretion via protein kinase A (PKA) pathway when cultured with GD and NGD granulose cells (mixed together) in vitro. 6. Western blot results showed decreased concentrations of cytochrome P450 side-chain cleavage (P450scc) enzyme, 3β-hydroxysteroid dehydrogenase (3β-HSD) enzyme and steroid acute regulatory (StAR) protein expression, when the cells were co-treated with EGF and oLH. 7. The inhibitory effect of oLH-induced P(4) production was attenuated by EGF by the addition of MAP-erk kinase (MEK) inhibitor (PD98059) suggests EGF may inhibit P(4) production by affecting via the mitogen-activated protein kinase (MAPK) pathway.

摘要

本研究旨在探讨表皮生长因子（EGF）对鸭卵巢颗粒细胞孕酮（P(4)）分泌的影响。
我们从泰雅鸭获得最大的（F1）卵泡，根据其与生殖盘的接近程度分离颗粒层并分离细胞。
体外培养颗粒细胞，分别用培养介质和细胞测定 P(4)和类固醇生成酶浓度。
与生殖盘远端（NGD）相比，靠近生殖盘（GD）的培养颗粒细胞中 P(4)浓度降低。
EGF 抑制基础和绵羊促黄体生成激素（oLH）诱导的 P(4)浓度。它还抑制了 P(4)的分泌通过蛋白激酶 A (PKA) 途径当培养与 GD 和 NGD 颗粒细胞（混合在一起）在体外。
Western blot 结果表明，当细胞同时用 EGF 和 oLH 处理时，细胞色素 P450 侧链裂解（P450scc）酶、3β-羟甾脱氢酶（3β-HSD）酶和类固醇急性调节蛋白（StAR）蛋白表达浓度降低。
通过添加丝裂原激活蛋白激酶（MAPK）途径的 MEK 抑制剂（PD98059），oLH 诱导的 P(4)产生的抑制作用减弱，表明 EGF 可能通过影响 MAPK 途径抑制 P(4)的产生。