Ackland J F, D'Agostino J, Ringstrom S J, Hostetler J P, Mann B G, Schwartz N B
Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208.
Biol Reprod. 1990 Aug;43(2):347-52. doi: 10.1095/biolreprod43.2.347.
We have measured changes in circulating immunoreactive (ir-) inhibin in male and female rats using an RIA with an antiserum raised against porcine inhibin alpha (1-26)-Gly-Tyr. The same synthetic peptide was used for standards and for the preparation of tracer. Serum ir-inhibin levels were significantly higher in intact female than in intact male rats (p less than 0.001). Immunoreactive inhibin was significantly reduced in both sexes 24 h after bilateral gonadectomy (p less than 0.0001). Unilateral ovariectomy (ULO) of female rats on metestrus caused a transient decrease in serum inhibin 8 h after surgery, but levels were not significantly different from those of sham-operated controls at later times after surgery. Increases in serum FSH and LH were observed for 8-18 h after ULO. Serum ir-inhibin levels were also measured on the early morning of estrus during the secondary FSH surge. At this time, ir-inhibin levels were low, while FSH levels were high and LH levels were low. These results show that serum ir-inhibin levels in rats are decreased at times when serum FSH levels are high.
我们使用一种放射免疫分析法（RIA），以针对猪抑制素α（1-26）-甘氨酸-酪氨酸产生的抗血清，测量了雄性和雌性大鼠循环中免疫反应性（ir-）抑制素的变化。相同的合成肽用于标准品和示踪剂的制备。完整雌性大鼠的血清ir-抑制素水平显著高于完整雄性大鼠（p<0.001）。双侧性腺切除术后24小时，两性的免疫反应性抑制素均显著降低（p<0.0001）。处于动情后期的雌性大鼠单侧卵巢切除（ULO）术后8小时，血清抑制素出现短暂下降，但术后较晚时间其水平与假手术对照组无显著差异。ULO术后8-18小时观察到血清促卵泡激素（FSH）和促黄体生成素（LH）升高。在第二次FSH高峰期间的动情期清晨也测量了血清ir-抑制素水平。此时，ir-抑制素水平较低，而FSH水平较高，LH水平较低。这些结果表明，在大鼠血清FSH水平较高时，血清ir-抑制素水平会降低。
