Lin Shu-Dian, Dai Ai-Guo, Xi Shou-Min
Department of Respiratory Medicine, Hunan Geriatric Hospital, Changsha 410001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2005 Aug;21(3):293-5.
To elucidate the location and effects of transcription factor-nuclear factor-kappaB (NF-kappaB) in lung tissues of rats with chronic obstructive pulmonary disease (COPD).
Fourteen male Wistar rats were randomly divided into COPD model and control groups equally. The COPD model was established by intratracheal instillation of lipopolysaccharide twice and exposure to cigarette smoke daily. We detected the NF-kappaB p65 protein in lung by immunohistochemical method, and the expression of NF-kappaB p65 mRNA in lung by in situ hybridization.
Immunohistochemistry, the expression of NF-kappaB p65 protein in alveolar, bronchiolar epithelium and arteriolar endothelium was significantly higher in the COPD group (0.426 +/- 0.007, 0.434 +/- 0.012 and 0.313 +/- 0.007, respectively) than those of the control group (0.115 +/- 0.006, 0.116 +/- 0.005 and 0.095 +/- 0.007, respectively, all P < 0.01). In situ hybridization showed that the expressions of NF-kappaB p65 mRNA in alveolar epithelium (0.203 +/- 0.008), bronchiolar and arteriolar smooth muscle cell (0.208 + 0. 010 and 0.206 + 0.007) of rats in the COPD group were stronger than those in the control group (0.100 +/- 0.006, 0.102 +/- 0.002 and 0.103 +/- 0.003 respectively) by semiquantitative analysis (all P < 0.01).
The expression and nuclear translocation of NF-kappaB may be the basis event of gene expression of many cytokines and inflammatory mediators, which may positively regulate gene expression of many cytokines and inflammatory mediators in various cell lines.
阐明转录因子核因子-κB（NF-κB）在慢性阻塞性肺疾病（COPD）大鼠肺组织中的定位及作用。
将14只雄性Wistar大鼠随机均分为COPD模型组和对照组。通过气管内两次滴注脂多糖并每日暴露于香烟烟雾建立COPD模型。采用免疫组化法检测肺组织中NF-κB p65蛋白，原位杂交法检测肺组织中NF-κB p65 mRNA的表达。
免疫组化显示，COPD组肺泡、细支气管上皮及小动脉内皮中NF-κB p65蛋白的表达（分别为0.426±0.007、0.434±0.012和0.313±0.007）显著高于对照组（分别为0.115±0.006、0.116±0.005和0.095±0.007，均P<0.01）。原位杂交显示，COPD组大鼠肺泡上皮（0.203±0.008）、细支气管及小动脉平滑肌细胞（0.208+0.010和0.206+0.007）中NF-κB p65 mRNA的表达通过半定量分析均强于对照组（分别为0.100±0.006、0.102±0.002和0.103±0.003，均P<0.01）。
NF-κB的表达及核转位可能是多种细胞因子和炎症介质基因表达的基础事件，可能正向调控多种细胞系中多种细胞因子和炎症介质的基因表达。
