Zheng Xia, Sun Jian, Hu Shen-Jiang, Zhu Zao-Hui, Wang Guo-Zhong, Li Jiang, Zhang Bi-Qi
First Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310003, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 May;22(2):142-6.
To investigate the changes in the expression of four kinds of calcium regulatory proteins mRNA on the isolated ischemia/ reperfusion (IR) hearts.
The rat hearts were divided into two groups: control group and IR group which received 45 min exposure to Krebs-Henseleit solution after 15 min zero-flow global ischemia. The indexes of left ventricular function, such as LVDP, +dp/dt(max), -dp/dt(max), and an arrhythmia scoring system were compared between the two groups. The messenger ribonucleic acid (mRNA) amount of sarcoplasmic reticulum (SR) Ca2+ ATPase (SERCA), phospholamban (PLB), inositol 1,4,5-trisphosphate receptor2 (IP3R2) and ryanodine receptor2 (RyR2) was measured by reverse transcription-polymerase chain reaction (RT-PCR) and normalized to the mRNA levels of beta-actin.
In the IR group, LVDP, +dp/dt(max) and -dp/dt(min) of the isolated hearts were depressed and the high rate of arrhythmias occurred during reperfusion. The levels of SERCA, IP3R2, RyR2 mRNA were lower in the IR isolated hearts group than those in the control group, while there was no difference in the level of phospholamban.
These data suggest that myocardial ischemia/reperfusion can induce the depression of cardiac performance and an increased risk of arrhythmias, concomitant with the decrease in SERCA, IP3R2, RyR2 mRNA steady state levels.
研究离体缺血/再灌注（IR）心脏中四种钙调节蛋白mRNA表达的变化。
将大鼠心脏分为两组：对照组和IR组，IR组在15分钟零流量全心缺血后接受45分钟的Krebs-Henseleit溶液灌注。比较两组左心室功能指标，如左心室舒张末压（LVDP）、最大上升速率（+dp/dt(max)）、最大下降速率（-dp/dt(max)）以及心律失常评分系统。通过逆转录-聚合酶链反应（RT-PCR）测量肌浆网（SR）Ca2+ATP酶（SERCA）、受磷蛋白（PLB）、肌醇1,4,5-三磷酸受体2（IP3R2）和兰尼碱受体2（RyR2）的信使核糖核酸（mRNA）量，并将其标准化为β-肌动蛋白的mRNA水平。
在IR组中，离体心脏的LVDP、+dp/dt(max)和-dp/dt(min)降低，再灌注期间心律失常发生率高。IR离体心脏组中SERCA、IP3R2、RyR2 mRNA水平低于对照组，而受磷蛋白水平无差异。
这些数据表明，心肌缺血/再灌注可导致心脏功能下降和心律失常风险增加，同时SERCA、IP3R2、RyR2 mRNA稳态水平降低。
