Li Chang-Chong, Ye Le-Ping, Su Miao-Shang, Hu Xiao-Guang, Zhang Wei-Xi, Luo Yun-Chun
Department of Respiratory Medicine,Yuying Chidren's Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2006 May;22(2):210-4.
To study the effect of achyranthes bidentata polysaccharides(ABPS) on the expression of signal transducer and activator of transcription 6 and its mRNA in bronchus of a rat model of asthma.
Thirty male SD rats were randomly divided into three groups: the control group, asthma group and ABPS group. The total cell numbers, eosinophils (EOS) numbers and differentiated cell numbers in bronchoalveolar lavage fluid (BALF) were counted by different count fluids. The concentrations of IL-4 in serum and BALF were measured by sandwich ELISA. The protein expressions of STAT6 were detected by immunohistochemistry techniques. The mRNA expressions of STAT6 were detected by hybridization in situ.
(1) The total cell numbers in BALF, the absolute numbers of EOS, the ratios of eosinophils to the total cell numbers (EOS%) of asthma group were all significantly higher than those of the control group (P < 0.01). The total cell numbers in BALF, the absolute numbers of EOS and EOS% of ABPS group were all significantly lower than those of asthma group (P < 0.01). (2) The concentrations of IL-4 in BALF and serum of asthma group were significantly higher than those of control group (P < 0.01), while the concentrations of IL-4 in BALF and serum of ABPS group were significantly lower than those of asthma group. (3) Immunohistochemistry showed that the protein content of STAT6 around the bronchus of asthma group was significantly higher than that of the control group (P < 0.01), while that of ABPS group was significantly lower than that of asthma group , the epithelial cells were the chief expression cells; hybridization in situ showed that the mRNA expression of STAT6 around the bronchus of asthma group was significantly higher than that of the control group (P < 0.01), while that of ABPS group was significantly lower than that of asthma group , the epithelial cells were the chief expression cells.
STAT6 protein and STAT6 mRNA were found strongly expressed in rat asthma model, the epithelial cells were the chief expression cells. ABPS had an inhibitory effect on airway inflammation cells infiltration such as EOS, it significantly depressed STAT6 and its mRNA expression, thus reduced the synthesis of IL-4 might be key in modulating mechanism of asthma.
研究牛膝多糖（ABPS）对哮喘大鼠模型支气管中信号转导和转录激活因子6（STAT6）及其mRNA表达的影响。
将30只雄性SD大鼠随机分为三组：对照组、哮喘组和ABPS组。用不同的计数液对支气管肺泡灌洗液（BALF）中的总细胞数、嗜酸性粒细胞（EOS）数和分化细胞数进行计数。用夹心ELISA法检测血清和BALF中IL-4的浓度。用免疫组织化学技术检测STAT6的蛋白表达。用原位杂交法检测STAT6的mRNA表达。
（1）哮喘组BALF中的总细胞数、EOS绝对数、嗜酸性粒细胞与总细胞数之比（EOS%）均显著高于对照组（P<0.01）。ABPS组BALF中的总细胞数、EOS绝对数和EOS%均显著低于哮喘组（P<0.01）。（2）哮喘组BALF和血清中IL-4的浓度显著高于对照组（P<0.01），而ABPS组BALF和血清中IL-4的浓度显著低于哮喘组。（3）免疫组织化学显示，哮喘组支气管周围STAT6的蛋白含量显著高于对照组（P<0.01），而ABPS组显著低于哮喘组，上皮细胞是主要表达细胞；原位杂交显示，哮喘组支气管周围STAT6的mRNA表达显著高于对照组（P<0.01），而ABPS组显著低于哮喘组，上皮细胞是主要表达细胞。
在大鼠哮喘模型中发现STAT6蛋白和STAT6 mRNA高表达，上皮细胞是主要表达细胞。ABPS对EOS等气道炎症细胞浸润有抑制作用，它显著抑制STAT6及其mRNA表达，从而减少IL-4的合成可能是调节哮喘机制的关键。
