蝎毒耐热蛋白对红藻氨酸诱导的原代大鼠海马神经肽Y能神经元损伤的影响
[Effects of scorpion venom heat-resistant protein on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons].
作者信息
Feng Yan-Hui, Yu De-Qin, Peng Yan, Yin Sheng-Ming, Sun Yi-Ping, Wu Xue-Fei, Zhang Wan-Qin, Zhao Jie
机构信息
Department of Physiology, Dalian Medical University, Dalian 116027, China.
出版信息
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Aug;23(3):315-8.
AIM
To investigate the effects of scorpion venom heat-resistant protein (SVHRP) on kainic acid induced-damage of cultured primitive rat hippocampal neuropeptide Y-nergic neurons.
METHODS
We observed morphological changes, celluar vigor, NPY-immunoreactivity and NPY mRNA expression by means of Thionine staining, MTT assay, immunocytochemistry and RT-PCR, respectively, on the primitively cultured Sprague-Dawley rat hippocampal neuron treated with KA and SVHRP for 24 h.
RESULTS
MTT assay and morphologic analysis showed that SVHRP markedly increased neuron survival-rate, and protected them from kA-induced damage. The expression of NPY-immunoreactivity and NPY mRNA in SVHRP group increased obviously compared with other groups.
CONCLUSION
SVHRP protected the primitively cultured hippocampal neurons from KA-induced neuroexcitotoxicity and promoted the expression of NPY.
目的
研究蝎毒耐热蛋白（SVHRP）对 kainic 酸诱导的原代培养大鼠海马神经肽 Y 能神经元损伤的影响。
方法
分别通过硫堇染色、MTT 法、免疫细胞化学和 RT-PCR 观察经 KA 和 SVHRP 处理 24 小时的原代培养 Sprague-Dawley 大鼠海马神经元的形态变化、细胞活力、NPY 免疫反应性和 NPY mRNA 表达。
结果
MTT 法和形态学分析表明，SVHRP 显著提高神经元存活率，并保护它们免受 KA 诱导的损伤。与其他组相比，SVHRP 组中 NPY 免疫反应性和 NPY mRNA 的表达明显增加。
结论
SVHRP 保护原代培养的海马神经元免受 KA 诱导的神经兴奋毒性，并促进 NPY 的表达。
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