Guo Fen, Wu Mei-Na, Jing Wei, Qi Jin-Shun
Department of Physiology, Shanxi Medical University, Taiyuan 030001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2007 Aug;23(3):381-4.
To study the feasibility of long-term potentiation(LTP) recording in the CA1 area of the rat in vivo with electrodes-binding technique.
Anesthetizing Wistar rats with urethane and fixing the animal on the stereotaxic device for acute surgery; implanting cannula into lateral cerebral ventricle; inserting self-made bound stimulating/recording electrodes into hippocampal CA1 area; recording basal field excitatory postsynaptic potential (fEPSP) and tetanus-induced long term potentiation (LTP).
fEPSPs were reliably induced by using the stimulating/recording electrodes-binding technique, and the appearance rate of fEPSP was nearly 100%; basal fEPSP recording was very stable, lasting for long time enough to finish all experiment; high frequency stimulation (HFS) successfully induced LTP, which maintained more than three hours, the inductivity is about 67%; paired-pulse facilitation (PPF) recording was also stable; intracerebroventricular (i c v) injection of amyloid beta suppressed HFSinduced LTP evidently.
The electrodes-binding technique for recording hippocampal LTP in vivo is quite simple and convenient. The experimental resource can be saved, and the rates of fEPSP appearance and LTP induction are kept high. Therefore, it is promising for this technique to be one electrophysiological auxiliary method in the research of learning and memory.
采用电极捆绑技术研究大鼠体内海马CA1区长期增强（LTP）记录的可行性。
用乌拉坦麻醉Wistar大鼠，将动物固定在立体定位装置上进行急性手术；将套管植入侧脑室；将自制的捆绑式刺激/记录电极插入海马CA1区；记录基础场兴奋性突触后电位（fEPSP）和强直刺激诱导的长期增强（LTP）。
使用刺激/记录电极捆绑技术可可靠地诱发出fEPSP，fEPSP的出现率近100%；基础fEPSP记录非常稳定，持续时间足够长以完成所有实验；高频刺激（HFS）成功诱导出LTP，其持续时间超过3小时，诱导率约为67%；双脉冲易化（PPF）记录也很稳定；脑室内注射β淀粉样蛋白明显抑制HFS诱导的LTP。
体内记录海马LTP的电极捆绑技术简单方便。可节省实验资源，fEPSP出现率和LTP诱导率保持较高。因此，该技术有望成为学习记忆研究中的一种电生理辅助方法。
