Chen Fang-min, Jiang Xi-nan, Shi Jia-qi, Yan Bo, Ren De-shuai, Gu Jiang, Li Deng-bao, Zhang Ya, Shen Jun, Liang Jun, Wang Yi
Department of Urology, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
Zhonghua Yi Xue Za Zhi. 2010 Nov 2;90(40):2864-8.
To study the influence and distribution in proximal tubule epithelial cells with the expressions of CD133 and CD34 in a rat model so as to provide a study basis for renal adult stem cell.
The kidney ischemia/reperfusion model was established by blocking the bilateral renal arteries for about 50 min and recovering the renal perfusion of blood. Then the rat kidneys were extracted at Days 3, 5 and 7 post-modeling. After a series of special treatment, immunohistochemical staining was used to show the distribution and expression intensity of KIM-1, Brdu antigen, CD34 and CD133 antigens in cells with the elapsing of time.
As compared with control group, the KIM-1 and CD133 antigens were present in cortex renis while the CD34 and Brdu antigens were distributed in parts of medulla renis and juncture of cortex-medulla renis. The expression density value of KIM-1 and CD133 antigens rose for the first 3 days then declined afterward (40.3% ± 3.2%, 57.5% ± 3.8%, 24.3% ± 1.4%). Otherwise the expression density value of CD 34 antigen declined (56.0% ± 4.8%, 44.2% ± 2.2%, 28.8% ± 1.0%) and Brdu antigen showed an upward trend at post-operation (10.0% ± 1.1%, 36.0% ± 4.2%, 48.8% ± 5.0%).
After ischemia/reperfusion injury in kidney, the expression rates of CD133 and KIM-1 antigen rise obviously in cortex renis. And the D34 and Brdu antigens show a similar trend in medulla renis. The result indicates that the phenomenon of proliferation and differentiation may appears in kidney proximal tubule and migrate from the region of medulla renis to cortex renis. The participating cells not only possess the strong proliferation and repairing ability of stem/progenitor cells, but also can expresses the CD34 and CD133 antigens. Thus it is may provide a study basis for the tissue reconstruction of nephron and research in the field of kidney adult stem cell.
通过大鼠模型研究CD133和CD34表达对近端肾小管上皮细胞的影响及其分布情况，为肾成体干细胞研究提供依据。
通过阻断双侧肾动脉约50分钟并恢复肾脏血液灌注建立肾脏缺血/再灌注模型。然后在建模后第3、5和7天提取大鼠肾脏。经过一系列特殊处理后，采用免疫组织化学染色观察随着时间推移KIM-1、Brdu抗原、CD34和CD133抗原在细胞中的分布及表达强度。
与对照组相比，KIM-1和CD133抗原存在于肾皮质，而CD34和Brdu抗原分布于部分肾髓质及肾皮质-髓质交界处。KIM-1和CD133抗原的表达密度值在第1个3天上升，之后下降（40.3%±3.2%，57.5%±3.8%，24.3%±1.4%）。否则，CD34抗原的表达密度值下降（56.0%±4.8%，44.2%±2.2%，28.8%±1.0%），Brdu抗原在术后呈上升趋势（10.0%±1.1%，36.0%±4.2%，48.8%±5.0%）。
肾脏缺血/再灌注损伤后，肾皮质中CD133和KIM-1抗原表达率明显升高。而D34和Brdu抗原在肾髓质呈现类似趋势。结果表明，肾脏近端小管可能出现增殖和分化现象，并从肾髓质区域迁移至肾皮质。参与的细胞不仅具有干细胞/祖细胞强大的增殖和修复能力，还能表达CD34和CD133抗原。因此，这可能为肾单位的组织重建及肾成体干细胞领域的研究提供依据。
