[宋内志贺氏菌2b型菌株中SfII和SfX原噬菌体的整合位点及排列研究]
[Study on the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains].
作者信息
Wang Yi-ting, Sun Qiang-zheng, Liu Kai, Zhao Ai-lan, Wang Yan, Jin Dong, Li Zhen-jun, Jing Huai-qi, Ye Chang-yun, Xu Jian-guo
机构信息
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2010 Jul;31(7):800-3.
OBJECTIVE
To study the integration site and arrangement of SfII and SfX prophages in Shigella flexneri serotype 2b strains.
METHODS
A series of primers were designed based on potential integration site of SfII and SfX prophages in Shigella flexneri serotype 2b strains, and PCR were performed for 50 serotype 2b strains to amplify special genes located in host and prophages. PCR products were sequenced to identify integration sites and arrangement of SfII and SfX.
RESULTS
In all the serotype 2b strains, prophage SfII and SfX were adjacent to each other, and integrated into the thrW tRNA gene of the host, which were located between genes proA and yaiC of host. Prophage SfX was located immediately upstream of prophage SfII in all the detected 50 serotype 2b strains exception for strain 51251.
CONCLUSION
This was the first report on the integration site and arrangement of serotype-converting prophages SfII and SfX in Shigella flexneri 2b strains.
目的
研究福氏志贺菌2b血清型菌株中SfII和SfX前噬菌体的整合位点及排列情况。
方法
根据福氏志贺菌2b血清型菌株中SfII和SfX前噬菌体的潜在整合位点设计一系列引物，对50株2b血清型菌株进行PCR，扩增宿主菌和前噬菌体中的特定基因。对PCR产物进行测序，以确定SfII和SfX的整合位点及排列情况。
结果
在所有2b血清型菌株中，前噬菌体SfII和SfX彼此相邻，并整合到宿主菌的thrW tRNA基因中，该基因位于宿主菌的proA和yaiC基因之间。在除51251菌株外的所有50株检测到的2b血清型菌株中，前噬菌体SfX位于前噬菌体SfII的紧邻上游。
结论
这是关于福氏志贺菌2b菌株中血清型转换前噬菌体SfII和SfX的整合位点及排列情况的首次报道。
Zotero 插件