Wang Yan-bin, Yang Zhao-xu, Cai Lei
Department of Gastroenterology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
Zhonghua Nei Ke Za Zhi. 2010 Oct;49(10):869-72.
To investigate the role of epithelial cell apoptosis in the stress-related changes of intestinal mucosa barrier following traumatic brain injury.
Sixty-four health male Wistar rats were divided randomly into two groups: (1) traumatic brain injury model group (n = 32), in which rats suffered from traumatic brain injury by Feeney's method; (2) control group (n = 32), rats suffered from sham operation. Each group were divided into four subgroups according 6 h, 12 h, 24 h, and 48 h after operation (n = 8, for each subgroup). Ileum tissue were taken to observed the damage of the intestinal mucosa under microscope and electron microscope. The early apoptosis rate of intestinal mucosal cells were analyzed with Annexin V-PI double stained and detected by flow cytometry.
The intestinal mucosa were damaged and the intercellular space of intestinal mucosal were found increased in traumatic brain injury group. The early apoptosis rate of intestinal mucosal epithelial cells was significant increased in traumatic brain injury group than that in control group [6 h: (13.5 ± 3.7)% vs (6.1 ± 1.7)%, P < 0.05; 12 h: (66.1 ± 6.0)% vs (5.2 ± 1.1)%, P < 0.05; 24 h: (39.8 ± 4.8)% vs (8.4 ± 2.6)%, P < 0.05; 48 h: (7.5 ± 1.3)% vs (6.6 ± 0.5)%].
The increased early apoptosis rate of intestinal mucosal epithelial cells might contribute to the stress-damage of the intestinal mucosa barrier in early stage of traumatic brain injury.
探讨上皮细胞凋亡在创伤性脑损伤后肠黏膜屏障应激相关变化中的作用。
将64只健康雄性Wistar大鼠随机分为两组：（1）创伤性脑损伤模型组（n = 32），采用Feeney法制作大鼠创伤性脑损伤模型；（2）对照组（n = 32），行假手术。每组再根据术后6 h、12 h、24 h和48 h分为4个亚组（每个亚组n = 8）。取小肠组织，在显微镜和电子显微镜下观察肠黏膜损伤情况。采用Annexin V-PI双染法分析肠黏膜细胞早期凋亡率，并通过流式细胞术进行检测。
创伤性脑损伤组肠黏膜受损，肠黏膜细胞间隙增宽。创伤性脑损伤组肠黏膜上皮细胞早期凋亡率显著高于对照组[6 h：（13.5±3.7）% vs（6.1±1.7）%，P < 0.05；12 h：（66.1±6.0）% vs（5.2±1.1）%，P < 0.05；24 h：（39.8±4.8）% vs（8.4±2.6）%，P < 0.05；48 h：（7.5±1.3）% vs（6.6±0.5）%]。
肠黏膜上皮细胞早期凋亡率增加可能是创伤性脑损伤早期肠黏膜屏障应激损伤的原因之一。
