Li Lin-wei, Yang Yang, Li Xiao-yan, Guo Li-ping, Zhou Yun, Lu Shi-xin
Department of Oncology, Henan Provincial People's Hospital, Zhengzhou 450003, China.
Zhonghua Yi Xue Za Zhi. 2010 Oct 19;90(38):2713-7.
To investigate the tumor-suppressing function of human esophageal cancer related gene 4 (ECRG4) in esophageal squamous cell carcinoma (ESCC).
The recombinant plasmid pcDNA3.1-ECRG4 with ECRG4 open reading frame was constructed. The EC9706 cell was transfected with either pcDNA3.1 or pcDNA3.1-ECRG4. And the effects of ECRG4 on tumor cell were examined by in vivo assays of cell proliferation, adhesion, migration, invasion and tumor growth. The expression levels of P53 and P21 proteins were detected in EC9706 cell with transfection of ECRG4 gene by Western blotting.
The final tumor volume and weight in ECRG4 transfection group were (264±43) mm3 and (0.39±0.09) g, versus (464±128) mm3 and (0.76±0.13) g in control group (both P<0.05). And the capacities of tumor cells adhesion, migration and invasion decreased in ECRG4 transfection group versus that in control group (all P>0.05). Furthermore, the expression levels of P53 and P21 proteins were higher in ECRG4 transfection group than those in control group (100.00±3.87, 35.71±2.36 vs 16.6±1.92, 1.09±0.11, both P<0.05).
As a novel candidate tumor suppressor in ESCC, ECRG4 may induce the up-regulation of p21 protein through p53 pathway to inhibit tumor growth in ESCC.
探讨人食管癌相关基因4（ECRG4）在食管鳞状细胞癌（ESCC）中的抑癌作用。
构建含ECRG4开放阅读框的重组质粒pcDNA3.1-ECRG4。将EC9706细胞分别用pcDNA3.1或pcDNA3.1-ECRG4转染。通过细胞增殖、黏附、迁移、侵袭及肿瘤生长的体内实验检测ECRG4对肿瘤细胞的影响。采用蛋白质免疫印迹法检测转染ECRG4基因的EC9706细胞中P53和P21蛋白的表达水平。
ECRG4转染组最终肿瘤体积和重量分别为（264±43）mm3和（0.39±0.09）g，对照组分别为（464±128）mm3和（0.76±0.13）g（均P<0.05）。与对照组相比，ECRG4转染组肿瘤细胞的黏附、迁移和侵袭能力降低（均P>0.05）。此外，ECRG4转染组P53和P21蛋白表达水平高于对照组（分别为100.00±3.87、35.71±2.36，对照组为16.6±1.92、1.09±0.11，均P<0.05）。
作为ESCC中一种新的候选抑癌基因，ECRG4可能通过p53途径诱导p21蛋白上调，从而抑制ESCC肿瘤生长。
