Mori Yoichiro, Ishiguro Hideyuki, Kuwabara Yoshiyuki, Kimura Masahiro, Mitsui Akira, Kurehara Horoki, Mori Ryota, Tomoda Keisuke, Ogawa Ryo, Katada Takeyasu, Harata Koshiro, Fujii Yoshitaka
Nagoya City University Graduate School of Medical Sciences, Oncology, Immunology and Surgery, Nagoya 467-8601, Japan.
Oncol Rep. 2007 Oct;18(4):981-5.
In this study, we examined the expression of esophageal cancer-related gene 4 (ECRG4) mRNA and evaluated its clinical significance in esophageal squamous cell carcinoma (ESCC). ECRG4 mRNA expression was quantified by real-time RT-PCR in 63 ESCC and corresponding normal esophageal mucosal samples. ECRG4 mRNA expression levels were significantly lower in ESCC tissues compared with corresponding normal esophageal mucosa (P<0.0001), in patients with locally invasive T2-4 tumors compared with less invasive T1 tumors (P=0.0229) and in stage 4 tumors compared with stage 0-3 tumors (P=0.0120). Furthermore, low ECRG4 mRNA expression levels were associated with significantly shorter survival after surgery compared with high ECRG4 mRNA expression levels (P=0.0150) in ESCC patients. On the basis of multivariate analysis, we conclude that ECRG4 mRNA expression level could be a candidate for an independent prognostic factor for ESCC patients.
在本研究中，我们检测了食管癌相关基因4（ECRG4）mRNA的表达，并评估了其在食管鳞状细胞癌（ESCC）中的临床意义。通过实时逆转录聚合酶链反应（RT-PCR）对63例ESCC组织及相应的正常食管黏膜样本中ECRG4 mRNA的表达进行定量分析。与相应的正常食管黏膜相比，ESCC组织中ECRG4 mRNA的表达水平显著降低（P<0.0001）；与浸润性较小的T1期肿瘤患者相比，局部浸润性T2-4期肿瘤患者中ECRG4 mRNA的表达水平较低（P=0.0229）；与0-3期肿瘤相比，4期肿瘤中ECRG4 mRNA的表达水平较低（P=0.0120）。此外，与ECRG4 mRNA高表达水平的ESCC患者相比，低表达水平的患者术后生存期显著缩短（P=0.0150）。基于多因素分析，我们得出结论，ECRG4 mRNA表达水平可能是ESCC患者独立预后因素的一个候选指标。
