Department of Orthopedics, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China.
Chin Med J (Engl). 2010 Nov;123(21):3055-60.
While attempting to restore bone stock, impaction bone grafting employed during revision joint surgery may result in slow and limited allograft incorporation into host bone. A new approach including gene-modified bone marrow stromal cells (BMSCs) in combination with impaction bone grafting may effectively restore bone stock and improve allograft incorporation. This study aimed to investigate the effect of impaction on gene-modified BMSCs seeded on granular bone allografts in vitro and in vivo.
Deep-frozen, granular, cancellous bone allografts from canines were prepared to serve as cell delivery scaffolds and were seeded with green fluorescent protein (GFP) genetically-modified BMSCs to construct cell-allograft composites. The composites were impacted in a simulative, in vitro impaction model and cultured for further analysis under standard conditions. Four Beagle dogs, treated with bilateral, uncemented proximal tibial joint hemiarthroplasty with a prosthesis, were implanted with autologous GFP gene-modified cell-allograft composites to repair the bone cavity around each prosthesis.
A significant reduction in cell viability was observed after impaction by fluorescence microscopy in vitro. However, there remained a proportion of GFP-positive cells that were viable and functionally active, as evidenced by the secretion of GFP protein in vitro and in vivo.
Gene-modified BMSCs seeded on granular allografts were able to withstand the impaction forces and to maintain their normal functions in vitro and in vivo, in spite of a partial loss in cell viability.
在进行翻修关节手术时，为了恢复骨量，采用打压植骨可能导致移植物缓慢且有限地与宿主骨结合。一种新的方法包括基因修饰的骨髓基质细胞（BMSCs）与打压植骨相结合，可能有效地恢复骨量并提高移植物的结合。本研究旨在探讨打压对体外和体内颗粒状同种异体骨移植中接种基因修饰的 BMSCs 的影响。
制备深冷冻、颗粒状、松质骨同种异体骨作为细胞输送支架，并将绿色荧光蛋白（GFP）基因修饰的 BMSCs 接种到构建细胞-移植物复合物中。将复合物在模拟的体外打压模型中进行打压，并在标准条件下培养进行进一步分析。将 4 只杂种犬进行双侧、非骨水泥性胫骨近端关节半关节置换术，植入自体 GFP 基因修饰的细胞-移植物复合物，以修复每个假体周围的骨腔。
体外荧光显微镜观察到打压后细胞活力明显下降。然而，仍有一部分 GFP 阳性细胞具有活力和功能活性，这可以通过体外和体内 GFP 蛋白的分泌得到证明。
尽管细胞活力部分丧失，但接种在颗粒状同种异体骨上的基因修饰的 BMSCs 能够承受打压力，并在体外和体内保持其正常功能。
