Wang Jin, Yin Jun-qiang, Jia Qiang, Shen Jing-nan, Huang Gang, Xie Xian-biao, Zou Chang-ye
Department of Orthopaedic Oncology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.
Zhonghua Zhong Liu Za Zhi. 2010 Oct;32(10):734-8.
To study the growth inhibition and apoptosis induction effects of bufalin on human osteosarcoma cell lines in vitro.
U-2OS and U-2OS/methotrexate (MTX) 300-resistant cell lines were treated with bufalin. Cell viability was assessed by MTT assay. Cell-cycle status, apoptosis-inducing effects, and the expression of apoptosis-related proteins were evaluated by flow cytometry, fluorescent staining, DNA fragmentation assay, and Western blotting.
Bufalin inhibited cell growth in both U-2OS and U-2OS/MTX300 cells. The IC(50) values of bufalin for U-2OS and U-2OS/MTX300 cells were (8.49 ± 2.1) ng/ml and (10.19 ± 1.7) ng/ml, respectively. The induction of G(2)/M cell-cycle arrest was also seen in the bufalin-treated cells. The bufalin-induced apoptosis was confirmed by increased expression of tumor suppressor protein p53, bax and decreased expression of bcl-2.
Bufalin inhibits the growth of and induces apoptosis in both MTX-sensitive and MTX-resistant human osteosarcoma U-2OS cell lines. The apoptosis-inducing effect of bufalin is not influenced by the presence of high levels of DHFR.
研究蟾毒灵对人骨肉瘤细胞系的体外生长抑制及诱导凋亡作用。
用蟾毒灵处理U-2OS和耐甲氨蝶呤（MTX）300的U-2OS细胞系。采用MTT法评估细胞活力。通过流式细胞术、荧光染色、DNA片段化分析和蛋白质印迹法评估细胞周期状态、诱导凋亡作用及凋亡相关蛋白的表达。
蟾毒灵抑制U-2OS和U-2OS/MTX300细胞的生长。蟾毒灵对U-2OS和U-2OS/MTX300细胞的IC(50)值分别为(8.49±2.1) ng/ml和(10.19±1.7) ng/ml。在经蟾毒灵处理的细胞中也观察到G(2)/M细胞周期阻滞的诱导。通过肿瘤抑制蛋白p53、bax表达增加及bcl-2表达降低证实了蟾毒灵诱导的凋亡。
蟾毒灵抑制MTX敏感和MTX耐药的人骨肉瘤U-2OS细胞系的生长并诱导其凋亡。蟾毒灵的诱导凋亡作用不受高水平二氢叶酸还原酶（DHFR）存在的影响。
