Strong M J, Svedmyr A, Gajdusek D C, Garruto R M
Laboratory of Central Nervous System Studies, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
Exp Neurol. 1990 Aug;109(2):171-9. doi: 10.1016/0014-4886(90)90071-y.
The subunit protein of the neurofibrillary tangle, the core protein of the neuritic plaque, and the amyloid of the cerebrovasculature in Alzheimer disease and normal aging is a unique 42-amino acid protein (amyloid beta-protein), suggesting a common origin for these pathological entities. However, the expression of the amyloid precursor protein mRNA (APP mRNA) from which the amyloid beta-protein is derived varies between specific neuronal populations. To determine the conditions under which neuronal synthesis of amyloid beta-protein might contribute to the formation of these structures, we have studied the temporal pattern of APP mRNA expression in developing fetal rabbit hippocampal neurons in vitro. Using in situ hybridization with a biotinylated riboprobe transcribed from a cDNA which includes the region encoding the amyloid beta-protein, we have observed a developmentally specific pattern of APP mRNA hybridization during neuronal maturation in vitro.
神经原纤维缠结的亚基蛋白、神经炎斑的核心蛋白以及阿尔茨海默病和正常衰老过程中脑血管淀粉样蛋白是一种独特的42个氨基酸的蛋白质（淀粉样β蛋白），这表明这些病理实体有共同的起源。然而，淀粉样β蛋白所源自的淀粉样前体蛋白mRNA（APP mRNA）在特定神经元群体中的表达有所不同。为了确定神经元合成淀粉样β蛋白可能有助于这些结构形成的条件，我们研究了体外培养的发育中胎兔海马神经元中APP mRNA表达的时间模式。使用与从包含编码淀粉样β蛋白区域的cDNA转录的生物素化核糖探针进行原位杂交，我们在体外神经元成熟过程中观察到了APP mRNA杂交的发育特异性模式。
